[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX), 200 Rxn

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Description 

The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.


Features

  • High Specificity

  • High Sensitivity

  • With ROX Reference Dye


Storage

Protect from light. 
Aliquot to avoid multiple freeze-thaw cycles. 

-20°C for 12 months

Odoo - Sample 1 for three columns

High Sensitivity

The amplification plot of real-time PCR with cDNA templates ranging from 76 fg to 20 ng in quantity, analyzed by using TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) for qPCR amplification. 

Odoo - Sample 2 for three columns

High Efficiency

The standard curve of TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) was generated by using 4-fold dilution of total cDNA ranging from 76 fg to 20 ng.

 

Contents

Component

Volume

Cat. No

ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

2 x 1 ml

TQ2110 (200 Rxn)


Storage 

Protected from light. 
Aliquot to avoid multiple freeze-thaw cycles.

-20°C for 12 months

 

Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328


Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells 

Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056

PMCID: PMC5522218


Hydroxylation and sulfation of sex steroid hormones in inflammatory liver 

Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

PMCID: PMC5706436

 

ExcelTaq™ 2X Q-PCR Master Mix

Product Name

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

SYBR

SYBR

SYBR

TaqMan

Blue contrast dye for best convenience

ü

ü

ü

ü

X

ROX dye for reference

X

ü

X

ü

ü

Time required for enzyme activation

10 min

10 min

2 min

2 min

10 min

qPCR program

Standard

Standard

Fast and Standard

Fast and Standard

Standard