[TQ1100] ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX), 200 RXN

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      Description 

      The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system. 


      Features

      • High sensitivity and signal intensity

      • Smart blue contrast dye as a visual aid for reaction setup

      • Better compatibility for reverse transcription

      • Low background 


      Storage 

      Protected from light.
      Aliquot to avoid multiple freeze-thaw cycles.

      -20°C for 12 months


      Odoo - Sample 1 for three columns

      Sensitivity

      The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.

      Odoo - Sample 2 for three columns

      Better Compatibility for reverse transcription 

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.


      Odoo - Sample 3 for three columns

      Specificity 

      The standard curve (left) and melting curve (right)  of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).



      Contents


      Component

      Volume 

            Cat. No

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      1 ml x 2

            TQ1100 (200 Rxn)

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      1 ml x 5

            TQ1101 (500 Rxn)


      Storage 

      Protected from light. 
      Aliquot to avoid multiple freeze-thaw cycles.

      -20°C for 12 months

       



      Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

      Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

      Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328


      Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

      Mohamad Javad Forouzani‐Moghaddam, Sina Habibi, Ahmad Hosseini‐Safa, Khadijeh Khanaliha, Roya Mokarinejad, Fatemeh Akhoundzadeh, Mojgan Oshaghi

      Vet Med Sci. 2024 May; 10(3): e1407. Published online 2024 Mar 22. doi: 10.1002/vms3.1407

      PMCID: PMC10959825


      Adiponectin affects the migration ability of bone marrow-derived mesenchymal stem cells via the regulation of hypoxia inducible factor 1α

      Sujung Soh, Sora Han, Hye In Ka, Se Hwan Mun, Woojung Kim, Gaeun Oh, Young Yang.   

      Cell Commun Signal. 2023; 21: 158. Published online 2023 Jun 27. doi: 10.1186/s12964-023-01143-y

      PMCID: PMC10294307


      Mitochondrial rewiring drives metabolic adaptation to NAD(H) shortage in triple negative breast cancer cells

      Agata Sofia Assuncao Carreira, Silvia Ravera, Chiara Zucal, Natthakan Thongon, Caffa Irene, Cecilia Astigiano, Nadia Bertola, Arianna Buongiorno, Michela Roccuzzo, Alessandra Bisio, Barbara Pardini, Alessio Nencioni, Santina Bruzzone, Alessandro Provenzani.    Neoplasia. 2023 Jul; 41: 100903. Published online 2023 May 4. doi: 10.1016/j.neo.2023.100903

      PMCID: PMC10192916


      Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency 

      Chiara Ambrosini, Eliana Destefanis, Eyemen Kheir, Francesca Broso, Federica Alessandrini, Sara Longhi, Nicolò Battisti, Isabella Pesce, Erik Dassi, Gianluca Petris, Anna Cereseto, Alessandro Quattrone

      Nucleic Acids Res. 2022 Oct 14; 50(18): 10756–10771. Published online 2022 Sep 27. doi: 10.1093/nar/gkac799

      PMCID: PMC9561285


      The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics

      Romina Monzani, Mara Gagliardi, Nausicaa Clemente, Valentina Saverio, Elżbieta Pańczyszyn, Claudio Santoro, Nissan Yissachar, Annalisa Visciglia, Marco Pane, Angela Amoruso, and Marco Corazzari

      Biology (Basel). 2022 Nov; 11(11): 1574. Published online 2022 Oct 27. doi: 10.3390/ biology11111574

      PMCID: PMC9687175


      The issue beyond resistance: Methicillin-resistant Staphylococcus epidermidis biofilm formation is induced by subinhibitory concentrations of cloxacillin, cefazolin, and clindamycin 

      Rasoul Mirzaei, Rasoul Yousefimashouf, Mohammad Reza Arabestani, Iraj Sedighi, Mohammad Yousef Alikhani

      PLoS One. 2022; 17(11): e0277287. Published online 2022 Nov 9. doi: 10.1371/ journal.pone.0277287

      PMCID: PMC9645612


      Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma 

      Lucia Longhitano, Nunzio Vicario, Stefano Forte, Cesarina Giallongo, Giuseppe Broggi, Rosario Caltabiano, Giuseppe Maria Vincenzo Barbagallo, Roberto Altieri, Giuseppina Raciti, Michelino Di Rosa, Massimo Caruso, Rosalba Parenti, Arcangelo Liso, Federica Busi, Marco Lolicato, Maria Caterina Mione, Giovanni Li Volti, Daniele Tibullo, Cancer Immunol Immunother. 2022 Jun 3. doi: 10.1007/s00262-022-03215-3. Online ahead of print.

      PMID: 35654889


      Prevention, inhibition, and degradation effects of melittin alone and in combination with vancomycin and rifampin against strong biofilm producer strains of methicillin-resistant Staphylococcus epidermidis

      Rasoul Mirzaei, Mohammad Yousef Alikhani, Carla Renata Arciola, Iraj Sedighi, Rasoul Yousefimashouf, Kamran Pooshang Bagheri, Biomed Pharmacother. 2022 Mar;147:112670. doi: 10.1016/j.biopha.2022.112670. Epub 2022 Feb 3.

      PMID: 35123230


      Gut Microbiota Modulation of Moderate Undernutrition in Infants through Gummy Lactobacillus plantarum Dad-13 Consumption: A Randomized Double-Blind Controlled Trial

      Rafli Zulfa Kamil, Agnes Murdiati, Mohammad Juffrie, Endang Sutriswati Rahayu, Nutrients. 2022 Mar 1;14(5):1049. doi: 10.3390/nu14051049.

      PMCID:  PMC8912314


      Integrative Metabolomic and Lipidomic Profiling of Lung Squamous Cell Carcinoma for Characterization of Metabolites and Intact Lipid Species Related to the Metastatic Potential

      Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Mostafa Sadeghi, Ali Reza Yousefi, Andrew Parks Benson, Poult Sci. 2021 Nov;100(11):101418. doi: 10.1016/j.psj.2021.101418. Epub 2021 Aug 8.

      PMCID:  PMC8531857


      Effects of coenzyme Q10 on reproductive performance of laying Japanese quail (Coturnix japonica) under cadmium challenge

      Heayyean Lee, Hwanhui Lee, Sujeong Park, Myeongsun Kim, Ji Young Park, Hanyong Jin, Kyungsoo Oh, Jeehyeon Bae, Young Yang, Hyung-Kyoon Choi, Cancers (Basel). 2021 Aug 19;13(16):4179. doi: 10.3390/cancers13164179.

      PMCID: PMC8391613


      Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

      Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore

      Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y

      PMCID: PMC6757040


      Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

      Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

      Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328


      Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

      Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

      Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328


      Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells 

      Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056

      PMCID: PMC5522218


      Hydroxylation and sulfation of sex steroid hormones in inflammatory liver 

      Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

      PMCID: PMC5706436


       ExcelTaq™ 2X Q-PCR Master Mix

      Product Name

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

      ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

      ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

      ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

      Cat. No.

      TQ1100

      TQ1110

      TQ1200

      TQ1210

      TQ2110

      Detection chemistry

      SYBR

      SYBR

      SYBR

      SYBR

      TaqMan

      Blue contrast dye for best convenience

      ü

      ü

      ü

      ü

      X

      ROX dye for reference

      X

      ü

      X

      ü

      ü

      Time required for enzyme activation

      10 min

      10 min

      2 min

      2 min

      10 min

      qPCR program

      Standard

      Standard

      Fast and Standard

      Fast and Standard

      Standard