Protein Marker Related Questions


Here are possible causes and solutions

A. May be masked by the prestained protein: extend the electrophoresis time to separate prestained protein from the low molecular weight bands.

B. Not suitable transfer membrane: try to use 0.22 μm PVDF.

C. Inappropriate methanol concentration: 

(a) Use freshly prepared transfer buffer. 

(b) Try to increasing the methanol concentration in the transfer buffer (up to 15% ~ 20% v/v methanol).