Real-time PCR Related Questions

 

 

Product Name



ExcelTaq™




2X Q-PCR





Master Mix


(SYBR, no




ROX)



ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

ExcelTa™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix

(SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix

(TaqMan, ROX)

ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX)

ExcelRT™ One-Step RT-qPCR Kit (TaqMan, no ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

RQ2110

RQ2200

Detection chemistry

SYBR

SYBR

SYBR

SYBR

TaqMan

TaqMan

TaqMan

Blue contrast dye for best convenience

V

V

V

V

X

X

X

Reference dye

No Rox

High Rox

No Rox

High Rox

High Rox

High Rox

No Rox

Time required for enzyme activation

10 min

10 min

2 min

2 min

10 min

3 min

3 min

qPCR program

Standard

Standard

Fast and Standard

Fast and Standard

Standard

Fast and Standard

Fast and Standard


Bio-Rad

CFX96™, CFX384™,

CFX Connect™

v

v

v

v

v

v

v

Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2

v

v

v

v

v

v

v

iCycler®, iQ™ 5, MyiQ™

v

v

v

v

v

v

v

Thermo Fisher (including Applied Biosystems and Life Technologies)

5700, 7000, 7300, StepOne™, StepOne™ plus

Δ

v

Δ

v

v

v

Δ

7500, 7500 FAST, QuantStudio™ 3, 5, 6, 7, 12k Flex, ViiA7™

Δ

Δ

Δ

Δ

Δ

Δ

Δ

7700, 7900, 7900HT,

7900HT FAST

Δ

v

Δ

v

v

v

Δ

Piko Real®

v

v

v

v

v

v

v

Qiagen (Corbett)

Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q

v

v

v

v

v

v

v

Roche

LightCycler® 480, LightCycler® 96, LightCycler® Nano

v

v

v

v

v

v

v

Agilent (Stratagene)

AriaMX, AriaDX

Δ

Δ

Δ

Δ

Δ

Δ

Δ

MX3000P®, MX3005P®, MX4000P®

Δ

Δ

Δ

Δ

Δ

Δ

Δ

BMS

Mic

v

v

v

v

v

v

v

BJS

Xxpress®

v

v

v

v

v

v

v

Cepheid

SmartCycler®

v

v

v

v

v

v

v

Eppendorf

Mastercycler® ep realplex 2S

v

v

v

v

v

v

v

Techne

PrimeQ, Quantica®

v

v

v

v

v

v

v

Fluidigm

BioMark™

Δ

Δ

Δ

Δ

Δ

Δ

Δ

Hain Lifescience

FluoroCycler® 96

v

v

v

v

v

v

v

 

IT-IS Life Science

MyGo Pro,

MyGo Mini

v

v

v

v

v

v

v

PCRmax

 

Eco™

v

v

v

v

v

v

v

 

Takara

Thermal Cycler Dice® (TP800)

v

v

v

v

v

v

v

V – Indicates the reagent is compatible to the instrument.
Δ – Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing.
*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.