Real-time PCR Related Questions

 

 

 

Product Name

ExcelTaq™ 2X Q-PCR Master Mix
(SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix
(SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix
(SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix
(SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix
(TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

SYBR

SYBR

SYBR

TaqMan

Blue contrast dye for best convenience

ü

ü

ü

ü

X

ROX dye for reference

X

ü

X

ü

ü

Time required for enzyme activation

10 min

10 min

2 min

2 min

10 min

qPCR program

Standard

Standard

Fast and Standard

Fast and Standard

Standard

Instrument Compatibility*

Applied Biosystems system:

   5700, 7000, 7300, 7700, and 7900HT

Δ

V

Δ

V

V

   StepOne, StepOnePlus

Δ

V

Δ

V

V

   7500, 7500 Fast, QuantStudio, ViiA7

Δ

Δ

Δ

Δ

Δ

BioRad system:

   CFX96 , CFX384

V

V

V

V

V

   iQ5

V

V

V

V

V

   Opticon, Opticon 2, Chromo 4

V

V

V

V

V

Cepheid system:

   Smart Cycler

V

V

V

V

V

Eppendorf system:

   Mastercycler ep realplex 

V

V

V

V

V

Roche system:

   LightCycler 480, Nano

V

V

V

V

V

QIAgen system:

   Rotor-Gene™ 3000, 6000, Q

V

V

V

V

V

Agilent (Stratagene) system:

   MX3000P, 3005P, 4000P

Δ

Δ

Δ

Δ

Δ

Illumina system:

   Eco

V

V

V

V

V

*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.
V– Indicates the reagent is compatible to the instrument
Δ– Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing.