Precast Gel Related Questions


1. Conventional Tris-Glycine running buffer is suitable for Q-PAGE™ TGN Precast Gels. 

2. 10X Tris-Glycine running buffer recipe: 

    Tris base 30.0 g, Glycine 144.0 g, SDS 10.0 g.

    Bring up the volume to 1 L with ddH2O and dilute to 1X for use.

Possible Cause:

 Incorrect running buffer

Suggested Solution:

A. Check buffer composition.

B. Use fresh 1X running buffer for inner and outer chamber.