[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 RXN

Call For Price

Description

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program.

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Features

High Stability
Fast Hot Start
High Sensitivity
Low Background
Suitable for Fast Program
Smart Blue Contrast Dye

Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months

Fast Hot Start

The overlapped amplification curves from different hot start duration display fast activation of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The initial activation step was set as 2,5, and 10 minutes. TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) contains a special hot-start Taq polymerase that omits time-consuming initial activation step required by chemically modified hot start Taq polymerases (Brand A).

High Sensitivity

The amplification curves from different amounts of cDNA display high sensitivity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The sensitivity assay of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) was examined with serial diluted cDNA from 25 ng to 1.4 fg as templates. The equal intervals of amplification curves also displayed the high precision of TQ1200 in target quantification.

Low Background

The single peak of melting curve displays high specificity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The post-amplification melting-curve analysis was examined with 5 ng cDNA as template, abnormally high concentration of primers (400 nM), and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) or qPCR master mix from brand A as reagents.

High Stability

The overlapped amplification curves and melting curves from different storage conditions display high stability of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

Stability was analyzed via real-time PCR amplification with 1 ng cDNA as template, and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) stored at -20°C, 4°C, 22°C and 37°C for 30 days as reagents.

Suitable for Fast Program

TQ1200 can be compatible with fast program and save 60% of run time (<1hr).

Smart Blue Contrast Dye

With smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.

Component

Volume

Cat. No

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

1 ml x 2

TQ1200 (200 Rxn)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

1 ml x 5

TQ1201 (500 Rxn)

Storage

Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months

Manual

Manual_TQ1200_ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) (2024 ver. 2.3.1)

SDS

SDS_TQ1200

Flyer

SMOBIO’s Q-PCR Master Mix- Instrument Compatibility

ExcelTaq™ 2X Fast Q-PCR Master Mix

TaqMan® vs. SYBR® Green Chemistry

How can I choose ExcelTaq™ 2X Q-PCR Master Mix?

What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?

Product Name

ExcelTaq™ 2X Q-PCR Master Mix
(SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix
(SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix
(SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix
(SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix
(TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

TaqMan

Blue contrast dye for best convenience

ROX dye for reference

Time required for enzyme activation

10 min

2 min

10 min

qPCR program

Standard

Fast and Standard

Standard

Instrument Compatibility*

Thermo Fisher (including Applied Biosystems system and Life Technologies):

5700, 7000, 7300, StepOne™, StepOne™ plus

7500, 7500 FAST, QuantStudio™ 1, 3, 5, 6, 7, 12k Flex, ViiA7™

7700, 7900, 7900HT,

7900HT FAST

Piko Real®

BioRad system:

CFX96™, CFX384™,

CFX Connect™

Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2

iCycler®, iQ™ 5, MyiQ™

Qiagen (Corbett):

Rotor-Gene™ 3000,

Rotor-Gene™ 6000,

Rotor-Gene™ Q

Roche system:

LightCycler® 480,

LightCycler® 96,

LightCycler® Nano

Agilent (Stratagene):

AriaMX, AriaDX

MX3000P®,

MX3005P®,

MX4000P®

BSM

Mic

BJS

Xxpress®

Cepheid

SmartCycler®

Eppendorf

Mastercycler® ep

realplex 2S

Fluidigm

BioMark™

Hain Lifescience

FluoroCycler® 96

IT-IS Life Science

MyGo Pro,

MyGo Mini

PCRmax

Eco™

Takara

Thermal Cycler

Dice®

TP8000

Techne

PrimeQ, Quantica®

Analytik Jena

qTOWER³

Bioevopeak

Real-time PCR system,

PCR-Q96 Series

EiNS Sci

E-QPCR4-CI,

E-QPCR6-CI

*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.

V– Indicates the reagent is compatible to the instrument

Δ– Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing.

Can you provide the results that using ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX, TQ1200) with different instruments?

Novel, soluble 3-heteroaryl-substituted tanshinone mimics attenuate the inflammatory response in murine macrophages.

Facen E, Assoni G, Donati G, Paladino D, Carreira A, Bonomo I, Pietra V, Lotti R, Houser J, Fava LL, Seneci P, Marinelli L, Arosio D, Provenzani A.

Sci Rep. 2024 Oct 18;14(1):24501. doi: 10.1038/s41598-024-73309-8.

PMCID: PMC11489580

Insights into the Sex-Related Effects of Dietary Polyphenols and Metabolic Disruptors on Inflammatory and (Neuro) Endocrine Pathways in Obesity: The HEAL Project.

Santangelo C, Scazzocchio B, Varì R, Ajmone-Cat MA, Tammaro A, Tait S, Masciola I, Tassinari R, Vincentini O, Di Benedetto R, Berry A, Cirulli F, Maranghi F, De Simone R, D'Archivio M.

Nutrients. 2024 Oct 23;16(21):3595. doi: 10.3390/nu16213595.

PMCID: PMC11547382

Correction: Risk assessment of transgender people: implementation of a demasculinizing-feminizing rodent model including the evaluation of thyroid homeostasis.

Tammaro A, Lori G, Martinelli A, Cancemi L, Tassinari R, Maranghi F.

Biol Direct. 2024 Jan 30;19(1):12. doi: 10.1186/s13062-024-00457-2.

PMCID: PMC10826112

Disease modeling by efficient genome editing using a near PAM-less base editor in vivo.

Rosello M, Serafini M, Mignani L, Finazzi D, Giovannangeli C, Mione MC, Concordet JP, Del Bene F.

Nat Commun. 2022 Jun 15;13(1):3435. doi: 10.1038/s41467-022-31172-z.

PMCID: PMC9198099

Zebrafish Melanoma-Derived Interstitial EVs Are Carriers of ncRNAs That Induce Inflammation.

Biagini V, Busi F, Anelli V, Kerschbamer E, Baghini M, Gurrieri E, Notarangelo M, Pesce I, van Niel G, D'Agostino VG, Mione M.

Int J Mol Sci. 2022 May 14;23(10):5510. doi: 10.3390/ijms23105510.

PMCID: PMC9143139

Insights into the Sex-Related Effects of Dietary Polyphenols and Metabolic Disruptors on Inflammatory and (Neuro) Endocrine Pathways in Obesity: The HEAL Project.

Santangelo C, Scazzocchio B, Varì R, Ajmone-Cat MA, Tammaro A, Tait S, Masciola I, Tassinari R, Vincentini O, Di Benedetto R, Berry A, Cirulli F, Maranghi F, De Simone R, D'Archivio M.

Nutrients. 2024 Oct 23;16(21):3595. doi: 10.3390/nu16213595.

PMCID: PMC11547382

Novel, soluble 3-heteroaryl-substituted tanshinone mimics attenuate the inflammatory response in murine macrophages.

Facen E, Assoni G, Donati G, Paladino D, Carreira A, Bonomo I, Pietra V, Lotti R, Houser J, Fava LL, Seneci P, Marinelli L, Arosio D, Provenzani A.

Sci Rep. 2024 Oct 18;14(1):24501. doi: 10.1038/s41598-024-73309-8.

PMCID: PMC11489580

Unraveling the Protective Role of Oleocanthal and Its Oxidation Product, Oleocanthalic Acid, against Neuroinflammation.

Barbalace MC, Freschi M, Rinaldi I, Zallocco L, Malaguti M, Manera C, Ortore G, Zuccarini M, Ronci M, Cuffaro D, Macchia M, Hrelia S, Giusti L, Digiacomo M, Angeloni C.

Antioxidants (Basel). 2024 Sep 3;13(9):1074. doi: 10.3390/antiox13091074.

PMCID: PMC11428454

Identification of Anti-Neuroinflammatory Bioactive Compounds in Essential Oils and Aqueous Distillation Residues Obtained from Commercial Varieties of Cannabis sativa L.

Barbalace MC, Freschi M, Rinaldi I, Mazzara E, Maraldi T, Malaguti M, Prata C, Maggi F, Petrelli R, Hrelia S, Angeloni C.

Int J Mol Sci. 2023 Nov 22;24(23):16601. doi: 10.3390/ijms242316601.

PMCID: PMC10706820

Risk Assessment of Transgender People: Development of Rodent Models Mimicking Gender-Affirming Hormone Therapies and Identification of Sex-Dimorphic Liver Genes as Novel Biomarkers of Sex Transition.

Tassinari R, Tammaro A, Lori G, Tait S, Martinelli A, Cancemi L, Frassanito P, Maranghi F.

Cells. 2023 Feb 1;12(3):474. doi: 10.3390/cells12030474.

PMCID: PMC9913858

Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats

Rochmy Istikharah, Dwi Aris Agung Nugrahaningsih, Ahmad Hamim Sadewa, Mae Sri Hartati Wahyuningsih

Malays J Med Sci. 2022 Jun;29(3):43-53. doi: 10.21315/mjms2022.29.3.5. Epub 2022 Jun 28.

PMCID: PMC9249416

Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells.

Kim O, Hwangbo C, Tran PT, Lee JH.

Cell Death Dis. 2022 Feb 8;13(2):122. doi: 10.1038/s41419-022-04594-2.

PMCID: PMC8826407

High CD169 Monocyte/Lymphocyte Ratio Reflects Immunophenotype Disruption and Oxygen Need in COVID-19 Patients.

Minutolo A, Petrone V, Fanelli M, Iannetta M, Giudice M, Ait Belkacem I, Zordan M, Vitale P, Rasi G, Sinibaldi-Vallebona P, Sarmati L, Andreoni M, Malergue F, Balestrieri E, Grelli S, Matteucci C.

Pathogens. 2021 Dec 18;10(12):1639. doi: 10.3390/pathogens10121639.

PMCID: PMC8715749

Evidence of the pathogenic HERV-W envelope expression in T lymphocytes in association with the respiratory outcome of COVID-19 patients.

Balestrieri E, Minutolo A, Petrone V, Fanelli M, Iannetta M, Malagnino V, Zordan M, Vitale P, Charvet B, Horvat B, Bernardini S, Garaci E, di Francesco P, Sinibaldi Vallebona P, Sarmati L, Grelli S, Andreoni M, Perron H, Matteucci C.

EBioMedicine. 2021 Apr;66:103341. doi: 10.1016/j.ebiom.2021.103341. Epub 2021 Apr 15.

PMCID: PMC8082064

UHRF1 Induces Methylation of the TXNIP Promoter and Down-Regulates Gene Expression in Cervical Cancer.

Kim MJ, Lee HJ, Choi MY, Kang SS, Kim YS, Shin JK, Choi WS.

Mol Cells. 2021 Mar 31;44(3):146-159. doi: 10.14348/molcells.2021.0001.

PMCID: PMC8019600

Cyclic RGD Pentapeptide Cilengitide Enhances Efficacy of Gefitinib on TGF-beta1-Induced Epithelial-to-Mesenchymal Transition and Invasion in Human Non-Small Cell Lung Cancer Cells.

Jeong J, Kim J.

Front Pharmacol. 2021 Mar 24;12:639095. doi: 10.3389/fphar.2021.639095. eCollection 2021.

PMCID: PMC8104086

Cyclic pentapeptide cRGDfK enhances the inhibitory effect of sunitinib on TGF-beta1-induced epithelial-to-mesenchymal transition in human non-small cell lung cancer cells.

Park KY, Kim J.

PLoS One. 2020 Aug 18;15(8):e0232917. doi: 10.1371/journal.pone.0232917. eCollection 2020.

PMCID: PMC7433881

Synthesis and Biological Evaluation of the Anti-Melanogenesis Effect of Coumaric and Caffeic Acid-Conjugated Peptides in Human Melanocytes.

Park KY, Kim J.

Front Pharmacol. 2020 Jun 17;11:922. doi: 10.3389/fphar.2020.00922. eCollection 2020.

PMCID: PMC7311773

Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells.

Lee Y, Jung JI, Park KY, Kim SA, Kim J.

Oncotarget. 2017 Jun 20;8(25):41091-41101. doi: 10.18632/oncotarget.17056.

PMCID: PMC5522218

Unraveling the Protective Role of Oleocanthal and Its Oxidation Product, Oleocanthalic Acid, against Neuroinflammation.

Barbalace MC, Freschi M, Rinaldi I, Zallocco L, Malaguti M, Manera C, Ortore G, Zuccarini M, Ronci M, Cuffaro D, Macchia M, Hrelia S, Giusti L, Digiacomo M, Angeloni C.

Antioxidants (Basel). 2024 Sep 3;13(9):1074. doi: 10.3390/antiox13091074.

PMCID: PMC11428454

Seed Halopriming: A Promising Strategy to Induce Salt Tolerance in Indonesian Pigmented Rice

Yekti Asih Purwestri, Siti Nurbaiti, Sekar Pelangi Manik Putri, Ignasia Margi Wahyuni, Siti Roswiyah Yulyani, Alfino Sebastian, Tri Rini Nuringtyas, Nobutoshi Yamaguchi

Plants (Basel). 2023 Aug 5;12(15):2879. doi: 10.3390/plants12152879.

PMCID: PMC10420915

Expression profile of HERVs and inflammatory mediators detected in nasal mucosa as a predictive biomarker of COVID-19 severity

Vita Petrone, Marialaura Fanelli, Martina Giudice, Nicola Toschi, Allegra Conti, Christian Maracchioni, Marco Iannetta, Claudia Resta, Chiara Cipriani, Martino Tony Miele, Francesca Amati, Massimo Andreoni, Loredana Sarmati, Paola Rogliani, Giuseppe Novelli, Enrico Garaci, Guido Rasi, Paola Sinibaldi-Vallebona, Antonella Minutolo, Claudia Matteucci, Emanuela Balestrieri, Sandro Grelli. Front Microbiol. 2023; 14: 1155624. Published online 2023 May 22. doi: 10.3389/fmicb.2023.1155624

PMCID: PMC10239953

Quantification of the Chemical Chaperone 4-Phenylbutyric Acid (4-PBA) in Cell Culture Media via LC-HRMS: Applications in Fields of Neurodegeneration and Cancer

Salvatore Villani, Giulia Dematteis, Laura Tapella, Mara Gagliardi, Dmitry Lim, Marco Corazzari, Silvio Aprile, Erika Del Grosso.

Pharmaceuticals (Basel). 2023 Feb; 16(2): 298. Published online 2023 Feb 14. doi: 10.3390/ph16020298

PMCID: PMC9960049

Cuticle Modifications and Over-Expression of the Chitin-Synthase Gene in Diflubenzuron-Resistant Phenotype

Valentina Lucchesi, Lorenzo Grimaldi, Valentina Mastrantonio, Daniele Porretta, Letizia Di Bella, Tania Ruspandini, Martino Luigi Di Salvo,3 John Vontas, Romeo Bellini, Agata Negri, Sara Epis, Silvia Caccia, Claudio Bandi, and Sandra Urbanelli

Insects. 2022 Dec; 13(12): 1109. Published online 2022 Nov 30. doi: 10.3390/insects13121109

PMCID: PMC9782986

Dapagliflozin attenuates diabetes-induced diastolic dysfunction and cardiac fibrosis by regulating SGK1 signaling

Seul-Gee Lee, Darae Kim, Jung-Jae Lee, Hyun-Ju Lee, Ro-kyung Moon, Yong-Joon Lee, Seung-Jun Lee, Oh-Hyun Lee, Choongki Kim, Jaewon Oh, Chan Joo Lee, Yong-ho Lee, Seil Park, Ok-Hee Jeon, Donghoon Choi, Geu-Ru Hong & Jung-Sun Kim, BMC Med. 2022 Sep 7;20(1):309. doi: 10.1186/s12916-022-02485-z.

PMID: 36068525

BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis

Marialuisa Casella, Gabriele Lori, Lucia Coppola, Cinzia La Rocca, Sabrina Tait. Int J Mol Sci. 2022 Nov; 23(22): 14465. Published online 2022 Nov 21. doi: 10.3390/ ijms232214465

PMCID: PMC9697228

Dapagliflozin attenuates diabetes-induced diastolic dysfunction and cardiac fibrosis by regulating SGK1 signaling

PMID: 36068525

The Gut-Ex-Vivo System (GEVS) Is a Dynamic and Versatile Tool for the Study of DNBS-Induced IBD in BALB/C and C57BL/6 Mice, Highlighting the Protective Role of Probiotics

Romina Monzani, Mara Gagliardi, Nausicaa Clemente, Valentina Saverio, Elżbieta Pańczyszyn, Claudio Santoro, Nissan Yissachar, Annalisa Visciglia, Marco Pane, Angela Amoruso, and Marco Corazzari

Biology (Basel). 2022 Nov; 11(11): 1574. Published online 2022 Oct 27. doi: 10.3390/ biology11111574

PMCID: PMC9687175

In Vitro Assessment and Toxicological Prioritization of Pesticide Mixtures at Concentrations Derived from Real Exposure in Occupational Scenarios

Sabrina Tait, Gabriele Lori, Roberta Tassinari, Cinzia La Rocca, Francesca Maranghi, Int J Environ Res Public Health. 2022 Apr 25;19(9):5202. doi: 10.3390/ijerph19095202.

PMCID: PMC9104687

Diterpene Resin Acids and Olefins in Calabrian Pine ( Pinus nigra subsp. laricio (Poiret) Maire) Oleoresin: GC-MS Profiling of Major Diterpenoids in Different Plant Organs, Molecular Identification and Expression Analysis of Diterpene Synthase Genes

Enrica Alicandri, Stefano Covino, Bartolomeo Sebastiani, Anna Rita Paolacci, Maurizio Badiani, Francesco Manti, Carmelo Peter Bonsignore, Agostino Sorgonà, Mario Ciaffi, Plants (Basel). 2021 Nov 5;10(11):2391. doi: 10.3390/plants10112391.

PMCID: PMC8622628

Anti-Inflammatory Effect for Atherosclerosis Progression by Sodium-Glucose Cotransporter 2 (SGLT-2) Inhibitor in a Normoglycemic Rabbit Model

Seul-Gee Lee, PhD,* Seung-Jun Lee, MD,* Jung-Jae Lee, BS, Jung-Sun Kim, MD, PhD, corresponding author Oh-Hyun Lee, MD, Choong-Ki Kim, MD, Darae Kim, MD, PhD, Yong-Ho Lee, MD, PhD, Jaewon Oh, MD, PhD, Seil Park, PhD, Ok-Hee Jeon, PhD, Sung-Jin Hong, MD, Chul-Min Ahn, MD, PhD, Byeong-Keuk Kim, MD, PhD, Young-Guk Ko, MD, PhD, Donghoon Choi, MD, PhD, Myeong-Ki Hong, MD, PhD, and Yangsoo Jang, MD, PhD, Korean Circ J. 2020 May; 50(5): 443–457. Published online 2020 Feb 12. doi: 10.4070/kcj.2019.0296

PMCID: PMC7098824

ExcelTaq™ 2X Q-PCR Master Mix

Product Name	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
Cat. No.	TQ1100	TQ1110	TQ1200	TQ1210	TQ2110
Detection chemistry	SYBR	SYBR	SYBR	SYBR	TaqMan
Blue contrast dye for best convenience	ü	ü	ü	ü	X
ROX dye for reference	X	ü	X	ü	ü
Time required for enzyme activation	10 min	10 min	2 min	2 min	10 min
qPCR program	Standard	Standard	Fast and Standard	Fast and Standard	Standard