[TQ1211] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 500 RXN

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Description 

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.


Features

  • High Stability

  • Fast Hot Start

  • High Sensitivity

  • Low Background / High Specificity 

  • Suitable for Fast Program 

  • Smart Blue Contrast Dye 

  • With ROX Reference Dye


Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles. 

-20°C for 12 months

Odoo - Sample 1 for three columns

Fast hot start and better efficiency

The standard curves were examined using fast program with cDNA ranging from 200 fg to 20 ng as template. The efficiency was calculated according to the slope of standard curve. The efficiency of TQ1210 is approximately 100% showing that TQ1210 is more compatible with fast program as compared to brand A (efficiency: 71.9).

        

High Sensitivity

The sensitivity assay of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) was examined with serially diluted cDNA from 25 ng to 1.4 fg as templates. The amplification curves display that TQ1210 is capable to amplify template as little as 1.4 fg, and over a wide range of template usage.


Odoo - Sample 3 for three columns

Low nonspecific product

The post-amplification melt-curve analyzed with relatively higher concentration of primers (400 nM) reveals the specificity of qPCR reagents. The multiple peaks of melt curve display a nonspecific and a target PCR products. The ratio of nonspecific PCR product to target PCR product represents the specificity of qPCR reagents. The ratio analyzed using TQ1210 (0.5 to 1) was lower than the ratio analyzed from brand P (2 to 1) and A (0.8 to 1), showing high specificity of TQ1210.

Odoo - Sample 1 for three columns

High Stability

Stability was analyzed via real-time PCR amplification with 2 ng, 20 pg and 0.2 pg cDNA as template, and TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) stored at -20°C or 37°C for 4 weeks as reagents. The overlapped amplification curves of qPCR reactions from different storage conditions display excellent stability of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX). Inconsistent Ct value and decreased fluorescent intensity were observed in qPCR master mix from brand P. 


Odoo - Sample 2 for three columns

Suitable for Fast Program

TQ1210 can be compatible with fast program and save 60% of run time (<1hr).

Odoo - Sample 3 for three columns

Smart Blue Contrast Dye

 With smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.

Contents


Component

Volume 

      Cat. No

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

 1 ml x 2

      TQ1210 (200 Rxn)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

1 ml x 5

      TQ1211 (500 Rxn)


Storage 

Protected from light. 
Aliquot to avoid multiple freeze-thaw cycles.

-20°C for 12 months

 



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 ExcelTaq™ 2X Q-PCR Master Mix

Product Name

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

SYBR

SYBR

SYBR

TaqMan

Blue contrast dye for best convenience

ü

ü

ü

ü

X

ROX dye for reference

X

ü

X

ü

ü

Time required for enzyme activation

10 min

10 min

2 min

2 min

10 min

qPCR program

Standard

Standard

Fast and Standard

Fast and Standard

Standard