![[TQ1210] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 200 RXN](/web/image/product.template/420/image?unique=bab062a)
Description
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Features
High Stability
Fast Hot Start
High Sensitivity
Low Background
Suitable for Fast Program
Smart Blue Contrast Dye
With ROX Reference Dye
Storage
Protect from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
Fast Hot start and better efficiency
The standard curves were examined using fast program with cDNA ranging from 200 fg to 20 ng as template. The efficiency was calculated according to the slope of standard curve. The efficiency of TQ1210 is approximately 100% showing that TQ1210 is more compatible with fast program as compared to brand A (efficiency: 71.9).
High Sensitivity
The sensitivity assay of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) was examined with serially diluted cDNA from 25 ng to 1.4 fg as templates. The amplification curves display that TQ1210 is capable to amplify template as little as 1.4 fg, and over a wide range of template usage.
Low nonspecific product
The post-amplification melt-curve analyzed with relatively higher concentration of primers (400 nM) reveals the specificity of qPCR reagents. The multiple peaks of melt curve display a nonspecific and a target PCR products. The ratio of nonspecific PCR product to target PCR product represents the specificity of qPCR reagents. The ratio analyzed using TQ1210 (0.5 to 1) was lower than the ratio analyzed from brand P (2 to 1) and A (0.8 to 1), showing high specificity of TQ1210.
High Stability
Stability was analyzed via real-time PCR amplification with 2 ng, 20 pg and 0.2 pg cDNA as template, and TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) stored at -20°C or 37°C for 4 weeks as reagents. The overlapped amplification curves of qPCR reactions from different storage conditions display excellent stability of TQ1210 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX). Inconsistent Ct value and decreased fluorescent intensity were observed in qPCR master mix from brand P.
Suitable for Fast Program
TQ1210 can be compatible with fast program and save 60% of run time (<1hr).
Smart Blue Contrast Dye
With smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Contents
Component | Volume | Cat. No |
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | 1 ml x 2 | TQ1210 (200 Rxn) |
ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | 1 ml x 5 | TQ1211 (500 Rxn) |
Storage
Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix | ExcelTaq™ 2X Q-PCR Master Mix | ExcelTaq™ 2X Fast Q-PCR Master Mix | ExcelTaq™ 2X Fast Q-PCR Master Mix | ExcelTaq™ 2X Q-PCR Master Mix | |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 | |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan | |
Blue contrast dye for best convenience | P | P | P | P | X | |
ROX dye for reference | X | P | X | P | P | |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min | |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard | |
Instrument Compatibility* | ||||||
Thermo Fisher (including Applied Biosystems system and Life Technologies): | ||||||
5700, 7000, 7300, StepOne™, StepOne™ plus | Δ | V | Δ | V | V | |
7500, 7500 FAST, QuantStudio™ 1, 3, 5, 6, 7, 12k Flex, ViiA7™ | Δ | Δ | Δ | Δ | Δ | |
7700, 7900, 7900HT, 7900HT FAST | Δ | V | Δ | V | V | |
Piko Real® | V | V | V | V | V | |
BioRad system: | ||||||
CFX96™, CFX384™, CFX Connect™ | V | V | V | V | V | |
Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2 | V | V | V | V | V | |
iCycler®, iQ™ 5, MyiQ™ | V | V | V | V | V | |
Qiagen (Corbett): | ||||||
Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | V | V | V | V | V | |
Roche system: | ||||||
LightCycler® 480, LightCycler® 96, LightCycler® Nano | V | V | V | V | V | |
Agilent (Stratagene): | ||||||
AriaMX, AriaDX | Δ | Δ | Δ | Δ | Δ | |
MX3000P®, MX3005P®, MX4000P® | Δ | Δ | Δ | Δ | Δ | |
BSM | ||||||
Mic | V | V | V | V | V | |
BJS | ||||||
Xxpress® | V | V | V | V | V | |
Cepheid | ||||||
SmartCycler® | V | V | V | V | V | |
Eppendorf | ||||||
Mastercycler® ep realplex 2S | V | V | V | V | V | |
Fluidigm | ||||||
BioMark™ | Δ | Δ | Δ | Δ | Δ | |
Hain Lifescience | ||||||
FluoroCycler® 96 | V | V | V | V | V | |
IT-IS Life Science | ||||||
MyGo Pro, MyGo Mini | V | V | V | V | V | |
PCRmax | ||||||
Eco™ | V | V | V | V | V | |
Takara | ||||||
Thermal Cycler Dice® | V | V | V | V | V | |
TP8000 | V | V | V | V | V | |
Techne | ||||||
PrimeQ, Quantica® | V | V | V | V | V | |
Analytik Jena | ||||||
qTOWER3 | V | V | V | V | V | |
Bioevopeak | ||||||
Real-time PCR system, PCR-Q96 Series | V | V | V | V | V | |
EiNS Sci | ||||||
E-QPCR4-CI, E-QPCR6-CI | V | V | V | V | V | |
Can you provide the results that using ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX, TQ1210) with different instruments?
Song GY, Kim SM, Back S, Yang SB, Yang YM.
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Zeinab Kabarkouhi, Sareh Arjmand, Seyed Omid Ranaei Siadat, Babak Shokri. Sci Rep. 2023; 13: 6797. Published online 2023 Apr 26. doi: 10.1038/s41598-023-34078-y
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ExcelTaq™ 2X Q-PCR Master Mix
Product Name | |||||
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |