Description
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and high signal intensity when compared with Brand A. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Features
High sensitivity and signal intensity
With ROX reference dye
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Storage
Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
High sensitivity
The amplification plot of real-time PCR with cDNA templates ranging from 1.49 fg to 25 ng in quantity, analyzed by using TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) for qPCR amplification.
Better fluorescent signal
SMOBIO’s TQ1110 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) shows better fluorescent signal as compared to a similar product (Brand A).
Contents
Component | Volume | Cat. No |
ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | 1 ml x 2 | TQ1110 (200 Rxn) |
Storage
Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix | ExcelTaq™ 2X Q-PCR Master Mix | ExcelTaq™ 2X Fast Q-PCR Master Mix | ExcelTaq™ 2X Fast Q-PCR Master Mix | ExcelTaq™ 2X Q-PCR Master Mix | |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 | |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan | |
Blue contrast dye for best convenience | P | P | P | P | X | |
ROX dye for reference | X | P | X | P | P | |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min | |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard | |
Instrument Compatibility* | ||||||
Thermo Fisher (including Applied Biosystems system and Life Technologies): | ||||||
5700, 7000, 7300, StepOne™, StepOne™ plus | Δ | V | Δ | V | V | |
7500, 7500 FAST, QuantStudio™ 1, 3, 5, 6, 7, 12k Flex, ViiA7™ | Δ | Δ | Δ | Δ | Δ | |
7700, 7900, 7900HT, 7900HT FAST | Δ | V | Δ | V | V | |
Piko Real® | V | V | V | V | V | |
BioRad system: | ||||||
CFX96™, CFX384™, CFX Connect™ | V | V | V | V | V | |
Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2 | V | V | V | V | V | |
iCycler®, iQ™ 5, MyiQ™ | V | V | V | V | V | |
Qiagen (Corbett): | ||||||
Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | V | V | V | V | V | |
Roche system: | ||||||
LightCycler® 480, LightCycler® 96, LightCycler® Nano | V | V | V | V | V | |
Agilent (Stratagene): | ||||||
AriaMX, AriaDX | Δ | Δ | Δ | Δ | Δ | |
MX3000P®, MX3005P®, MX4000P® | Δ | Δ | Δ | Δ | Δ | |
BSM | ||||||
Mic | V | V | V | V | V | |
BJS | ||||||
Xxpress® | V | V | V | V | V | |
Cepheid | ||||||
SmartCycler® | V | V | V | V | V | |
Eppendorf | ||||||
Mastercycler® ep realplex 2S | V | V | V | V | V | |
Fluidigm | ||||||
BioMark™ | Δ | Δ | Δ | Δ | Δ | |
Hain Lifescience | ||||||
FluoroCycler® 96 | V | V | V | V | V | |
IT-IS Life Science | ||||||
MyGo Pro, MyGo Mini | V | V | V | V | V | |
PCRmax | ||||||
Eco™ | V | V | V | V | V | |
Takara | ||||||
Thermal Cycler Dice® | V | V | V | V | V | |
TP8000 | V | V | V | V | V | |
Techne | ||||||
PrimeQ, Quantica® | V | V | V | V | V | |
Analytik Jena | ||||||
qTOWER3 | V | V | V | V | V | |
Bioevopeak | ||||||
Real-time PCR system, PCR-Q96 Series | V | V | V | V | V | |
EiNS Sci | ||||||
E-QPCR4-CI, E-QPCR6-CI | V | V | V | V | V |
Can you provide the results that using ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX, TQ1110) with different instruments?
Zahra Khosrowpour, Seyed Mahmoud Hashemi, Samira Mohammadi-Yeganeh, Sara Simorgh, Behnaz Sadat Eftekhari, Peiman Brouki Milan, Subhas C. Kundu, Mazaher Gholipourmalekabadi
ACS Omega. 2024 Feb 6; 9(5): 5298–5318. Published online 2024 Jan 25. doi: 10.1021/acsomega.3c06175
PMCID: PMC10851416
Estrone-Based Derivatives Stabilize the c-MYC and c-KIT G-Quadruplex DNA Structures
Satendra Kumar, Annyesha Biswas, Sruthi Sudhakar, Divya Kumari, Pushpangadan Indira Pradeepkumar
ACS Omega. 2024 Feb 13; 9(6): 6616–6626. Published online 2024 Jan 29. doi: 10.1021/acsomega.3c07574
PMCID: PMC10870291
Alieh Farshbaf, Farnaz Mohajertehran, Seyed Hamid Aghaee‐Bakhtiari, Hossein Ayatollahi, Katayoun Douzandeh, Atessa Pakfetrat, Nooshin Mohtasham
Clin Exp Dent Res. 2024 Apr; 10(2): e877. Published online 2024 Mar 13. doi: 10.1002/cre2.877
PMCID: PMC10938069
YUSTINA CAROLINA FEBRIANTI SALSINHA, SITI NURBAITI, ALFINO SEBASTIAN,DIDIK INDRADEWA, YEKTI ASIH PURWESTRI, DIAH RACHMAWATI
BIODIVERSITAS 2022 July Volume 23, Number 7, Pages: 3573-3583
Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056
PMCID: PMC5522218
ExcelTaq™ 2X Q-PCR Master Mix
Product Name | |||||
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |