Description
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
Features
High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background
Storage
Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
Sensitivity
The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.
Better Compatibility for reverse transcription
ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.
Specificity
The standard curve (left) and melting curve (right) of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).
Contents
Component |
Volume |
Cat. No |
ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) |
1 ml x 2 |
TQ1100 (200 Rxn) |
ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) |
1 ml x 5 |
TQ1101 (500 Rxn) |
Storage
Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix | ExcelTaq™ 2X Q-PCR Master Mix | ExcelTaq™ 2X Fast Q-PCR Master Mix | ExcelTaq™ 2X Fast Q-PCR Master Mix | ExcelTaq™ 2X Q-PCR Master Mix | |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 | |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan | |
Blue contrast dye for best convenience | P | P | P | P | X | |
ROX dye for reference | X | P | X | P | P | |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min | |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard | |
Instrument Compatibility* | ||||||
Thermo Fisher (including Applied Biosystems system and Life Technologies): | ||||||
5700, 7000, 7300, StepOne™, StepOne™ plus | Δ | V | Δ | V | V | |
7500, 7500 FAST, QuantStudio™ 1, 3, 5, 6, 7, 12k Flex, ViiA7™ | Δ | Δ | Δ | Δ | Δ | |
7700, 7900, 7900HT, 7900HT FAST | Δ | V | Δ | V | V | |
Piko Real® | V | V | V | V | V | |
BioRad system: | ||||||
CFX96™, CFX384™, CFX Connect™ | V | V | V | V | V | |
Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2 | V | V | V | V | V | |
iCycler®, iQ™ 5, MyiQ™ | V | V | V | V | V | |
Qiagen (Corbett): | ||||||
Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q | V | V | V | V | V | |
Roche system: | ||||||
LightCycler® 480, LightCycler® 96, LightCycler® Nano | V | V | V | V | V | |
Agilent (Stratagene): | ||||||
AriaMX, AriaDX | Δ | Δ | Δ | Δ | Δ | |
MX3000P®, MX3005P®, MX4000P® | Δ | Δ | Δ | Δ | Δ | |
BSM | ||||||
Mic | V | V | V | V | V | |
BJS | ||||||
Xxpress® | V | V | V | V | V | |
Cepheid | ||||||
SmartCycler® | V | V | V | V | V | |
Eppendorf | ||||||
Mastercycler® ep realplex 2S | V | V | V | V | V | |
Fluidigm | ||||||
BioMark™ | Δ | Δ | Δ | Δ | Δ | |
Hain Lifescience | ||||||
FluoroCycler® 96 | V | V | V | V | V | |
IT-IS Life Science | ||||||
MyGo Pro, MyGo Mini | V | V | V | V | V | |
PCRmax | ||||||
Eco™ | V | V | V | V | V | |
Takara | ||||||
Thermal Cycler Dice® | V | V | V | V | V | |
TP8000 | V | V | V | V | V | |
Techne | ||||||
PrimeQ, Quantica® | V | V | V | V | V | |
Analytik Jena | ||||||
qTOWER3 | V | V | V | V | V | |
Bioevopeak | ||||||
Real-time PCR system, PCR-Q96 Series | V | V | V | V | V | |
EiNS Sci | ||||||
E-QPCR4-CI, E-QPCR6-CI | V | V | V | V | V |
Can you provide the results that using ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX, TQ1100) with different instruments?
Mohamad Javad Forouzani‐Moghaddam, Sina Habibi, Ahmad Hosseini‐Safa, Khadijeh Khanaliha, Roya Mokarinejad, Fatemeh Akhoundzadeh, Mojgan Oshaghi
Vet Med Sci. 2024 May; 10(3): e1407. Published online 2024 Mar 22. doi: 10.1002/vms3.1407
PMCID: PMC10959825
Lucia Longhitano, Nunzio Vicario, Stefano Forte, Cesarina Giallongo, Giuseppe Broggi, Rosario Caltabiano, Giuseppe Maria Vincenzo Barbagallo, Roberto Altieri, Giuseppina Raciti, Michelino Di Rosa, Massimo Caruso, Rosalba Parenti, Arcangelo Liso, Federica Busi, Marco Lolicato, Maria Caterina Mione, Giovanni Li Volti, Daniele Tibullo, Cancer Immunol Immunother. 2022 Jun 3. doi: 10.1007/s00262-022-03215-3. Online ahead of print.
PMID: 35654889
Rasoul Mirzaei, Mohammad Yousef Alikhani, Carla Renata Arciola, Iraj Sedighi, Rasoul Yousefimashouf, Kamran Pooshang Bagheri, Biomed Pharmacother. 2022 Mar;147:112670. doi: 10.1016/j.biopha.2022.112670. Epub 2022 Feb 3.
PMID: 35123230
Rafli Zulfa Kamil, Agnes Murdiati, Mohammad Juffrie, Endang Sutriswati Rahayu, Nutrients. 2022 Mar 1;14(5):1049. doi: 10.3390/nu14051049.
PMCID: PMC8912314
Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Mostafa Sadeghi, Ali Reza Yousefi, Andrew Parks Benson, Poult Sci. 2021 Nov;100(11):101418. doi: 10.1016/j.psj.2021.101418. Epub 2021 Aug 8.
PMCID: PMC8531857
Heayyean Lee, Hwanhui Lee, Sujeong Park, Myeongsun Kim, Ji Young Park, Hanyong Jin, Kyungsoo Oh, Jeehyeon Bae, Young Yang, Hyung-Kyoon Choi, Cancers (Basel). 2021 Aug 19;13(16):4179. doi: 10.3390/cancers13164179.
PMCID: PMC8391613
Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore
Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y
PMCID: PMC6757040
Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore
Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328
Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056
PMCID: PMC5522218
Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031
PMCID: PMC5706436
ExcelTaq™ 2X Q-PCR Master Mix
Product Name |
|||||
Cat. No. |
TQ1100 |
TQ1110 |
TQ1200 |
TQ1210 |
TQ2110 |
Detection chemistry |
SYBR |
SYBR |
SYBR |
SYBR |
TaqMan |
Blue contrast dye for best convenience |
ü |
ü |
ü |
ü |
X |
ROX dye for reference |
X |
ü |
X |
ü |
ü |
Time required for enzyme activation |
10 min |
10 min |
2 min |
2 min |
10 min |
qPCR program |
Standard |
Standard |
Fast and Standard |
Fast and Standard |
Standard |