[CC5202] Champion™ DH5α High, 80 tubes (Region Limited)

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General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.


Kit contents

  1. Champion™ Competent Cells

  2. pUC19 Control Plasmid (5 μl, 10-4 μg/μl)

  3. Champion™ Transformation Protocol Card


Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 


Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.


 

Genotypes and applications

Product Name

Genotype

Application

Champion™ 109 High

e14(McrA-)recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 thi-1 relA1 ∆(lac-proAB) (F’traD36 proAB lacIqZ∆M15

Appropriate for blue-white color and robotic screening. It is a fast growing strain forming visible colonies within 8~10 hours.

Champion™ 21

F’ ompT hsdSβ(rβ-mβ-) dcm gal λ (DE3)

Appropriate host for recombinant protein expression using T7-based expression vectors.

Champion™ DH5α High

recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 relA1 thi-1 ∆(lacZYA-argF)U 169 φ80 ∆(lacZ)M15 F-

Suitable for cloning with large plasmid and cDNA library construction, and also for blue-white colony selection.



Items and ordering information


Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

 >1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion™ 21

E. coli BL21 (DE3)

 >1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion™ DH5α High

E. coli DH5α

 >3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

 




Odoo image and text block

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

 >1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion 21

E. coli BL21 (DE3)

 >1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion DH5α High

E. coli DH5α

 >3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

 

Odoo - Sample 1 for three columns

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

 

Strategic targeting of Cas9 nickase induces large segmental duplications.

Sugiyama Y, Okada S, Daigaku Y, Kusumoto E, Ito T.                     Cell Genom. 2024 Aug 14;4(8):100610. doi: 10.1016/j.xgen.2024.100610. Epub 2024 Jul 24.

PMCID: PMC11406185


Patient-derived and gene-edited pluripotent stem cells lacking NPHP1 recapitulate juvenile nephronophthisis in abnormalities of primary cilia and renal cyst formation.

Arai Y, Ito H, Shimizu T, Shimoda Y, Song D, Matsuo-Takasaki M, Hayata T, Hayashi Y.

Front Cell Dev Biol. 2024 Jun 26;12:1370723. doi: 10.3389/fcell.2024.1370723. eCollection 2024.

PMCID: PMC11233770