[CK1000] Champion™ E. coli Transformation Kit, 200 Rxn

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Description 

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–10cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 


Features

  • Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation. 

  • Fast and Easy– only few steps for preparation; suitable for time-saving transformation 

  • High efficiency– up to 109 cfu/μg 

  • Personalization– suitable for most E. coli strains


Kit Contents






Component

Volume






Champion™ CC Buffer

20 ml






SMO-Broth™

2 x 100 ml






pUC19 Control Plasmid (10-4 μg/μl)

5 µl






Instruction Manual

1






Champion™ Competent Cell Preparation Card

1


Storage

4°C for 12 months

 
 

Prepare Competent Cells with High Transformation Efficiency Using Champion™ E.coli Transformation Kit

 

 
 

Prepare Competent Cells Directly from E.coli Colonies Using Champion™ E.coli Transformation Kit

 

 
 

Time-saving Transformation Protocol


 Kit Contents

Component

Volume

Champion™ CC Buffer

20 ml

SMO-Broth™

2 x 100 ml

pUC19 Control Plasmid (10-4 μg/μl)

5 µl

Instruction Manual

1

Champion™ Competent Cell Preparation Card

1

Storage

4°C for 12 months


Using proper antibiotics of suitable concentration or using fresh antibiotics. 

 


 


Odoo - Sample 1 for three columns

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

Odoo - Sample 3 for three columns

Colony PCR

Analyze colonies with PCR master mix to save preparation time.