[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)

Call For Price




 

Description 

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products. 


Features

  • 5'→3' DNA polymerase activity

  • 3'→5' exonuclease activity (proofreading)

  • High reaction rate (up to 1 kb/10 second)

  • High fidelity, 70 times higher than Taq DNA polymerase 

  • Blunt end amplicon

  • Thermo-stable-half life is more than 10 hrs at 95°C 


Storage

[TF1000] SMO-HiFi™ DNA Polymerase

-20°C for 24 months


Odoo - Sample 1 for three columns

Elongation capability

SMO-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 12 kb in length (M: DM5100). 

Odoo - Sample 2 for three columns

Sensitivity

SMO-HiFi™ DNA Polymerase can amplify PCR products from as little as 1 fg of template DNA (M: DM2000). 

  

[TF1000] SMO-HiFi™ DNA Polymerase

Contents

Component

Volume

SMO-HiFi™ DNA Polymerase (1 U/μl)

100 μl

10X HiFi™ Buffer

600 μl

25 mM MgSO4

500 μl

dNTPs Mix (2 mM each)

600 μl

DMSO

600 μl


Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol


Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 


Storage

-20°C for 24 months 


 

[TF1000] SMO-HiFi™ DNA Polymerase

Manual

Manual_TF1000_SMO-HiFi™ DNA Polymerase

SDS

SDS_TF1000


SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000) contain different high fidelity DNA polymerases. SMO-HiFi™ (TF1000) and G-HiFi™(TF3000) DNA polymerases are suitable for GC-rich templates that are difficult to amplify.  Moreover G-HiFi™  (TF3000) is blended with elongation enhancer to perform quick and accurate PCR. 

 


 

Recommended PCR Condition 

[TF1000] SMO-HiFi™ DNA Polymerase

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

10X HiFi Buffer

5 µl

MgSO4 (25 mM)

2 μl

dNTPs (2 mM each)

5 µl

SMO-HiFi DNA Polymerase

1 µl (1 unit)

DMSO

5 µl

H2O

to 50 µl

Total volume

50 µl

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C

30 sec

Extension

68°C

30 sec/kb

Final extension

68°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties. 

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

Odoo - Sample 3 for three columns

Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.

Odoo - Sample 3 for three columns

Colony PCR

Analyze colonies with PCR master mix to save preparation time.