B-BOX™ Blue Light LED Epi-illuminator

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Description 

SMOBIO’s B-BOX™ is a long wavelength, blue light LED epi-illuminator. It is compact in design and robust in structure. The B-BOX™ epi-illuminator provides an unprecedented level of safety for its user due to its non-UV light source and a low operating voltage of only 12 Volts, as well as its capability working with non-carcinogenic DNA/ protein dye. The B-BOX™ comes with high quality non-flickering LED light that is highly sensitive for DNA and protein dye. B-BOX™ can greatly ease the routine chore of gel extraction, enabling easy visualization and gel cutting even in bright ambient light. The multi-angle filter plate (Cat. No. VE0102) provides optimal angles for gel cutting, visualization, and documentation. The B-BOX™ also comes with amber colored filter goggles (Cat. No. VE0103) for gel cutting and visualization. The Phox™ Photobox (Cat. No. VE0104) provides a mini darkroom environment for images taken with any smartphone. Finally, a built-in barrier system around the working area helps facilitate cleaning.


Features

  • Improved cloning efficiency 

  • Compact, lightweight, and portable (less than 1 kg in weight)

  • Safety features include : 470 nm long wavelength, without any UV radiation hazard to its user

  • Compatible with non-carcinogenic, non-ethidium bromide DNA staining dye

  • User friendly: Samples are easy to visualize (when using the filter plate or goggles) 

  • LED light source lasts up to 50,000 hours

  • Superior detection sensitivity: ≤ 0.04 ng of DNA when using FluoroStain™ DNA Fluorescent Staining Dye, ≤3 ng of protein when using FluoroStain™ Protein Fluorescent Staining Dye (as sensitive as silver stain)

  • Adjustable and removable filter plate allows for gel cutting, visualization, and documentation

  • Built-in barrier design, for easy clean up

  • Visible in bright ambient light

  • Emphasizes minimal power reliance, low heat generation, with its own built-in heat sink


Accessories

B-BOX™ Blue Light LED Epi-illuminator, DC 12 Volt, 0.72 Amp

x1

Adapter, AC 100-240V, 50/60 Hz, 1.8 m Power Cord

x1

Multi-angle Filter Plate for B-BOX™

x1

Amber Goggles for B-BOX™

x1

Phox™ Photobox

x1

Instruction Manual

x1

Warranty Card       

x1


Odoo - Sample 1 for three columns

Wavelength of Blue light LED (nm)

 

 
 

Advantages of using B-BOX™ Blue Light LED Epi-illuminator


 

 
 

SMOBIO's Phox™ Photobox assembly


Odoo - Sample 1 for three columns

Multiple angle filter plate

 

Odoo - Sample 2 for three columns

Easily accessible operating area

 

Odoo - Sample 3 for three columns

DNA/Protein bi-functional


Odoo - Sample 3 for three columns

100X increased cloning efficiency


 Physical Specifications

Overall Dimension (mm)

201.4 x 200 x 38 (D x W x H)

Viewing Area (mm)

158 x 96 (D x W)

Wavelength of LED (nm)

470

Number of LED Unit

72 Super Flux LED

Power

DC 12 Volt, 0.72 Amp

Electrical Requirements

AC 100~240 V, 50/60 Hz (Adapter)

Weight (kg)

0.95 (Net Weight)

Shipping Weight (kg)

1.0 (Gross Weight) – Adapter (0.5 kg) not included

Material:

ASA for Housing; Tempered Glass for Work Platform

Dyes Recommended

[DS1000] FluoroStain™ DNA Fluorescent Staining Dye 

[NS1000] FluoroVue™ Nucleic Acid Gel Stain 

[DL5000] FluoroDye™ DNA Fluorescent Loading Dye 

[PS1000] FluoroStain™ Protein Fluorescent Staining Dye 

SYBR Green I Nucleic Acid Gel Stain



Accessories


B-BOX™ Blue Light LED Epi-illuminator, DC 12 Volt, 0.72 Amp

x1

Adapter, AC 100-240V, 50/60 Hz, 1.8 m Power Cord

x1

Multi-angle Filter Plate for B-BOX™

x1

Amber Goggles for B-BOX™

x1

Phox™ Photobox

x1

Instruction Manual

x1

Warranty Card       

x1




 


 

Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium

Gabriella Chong, Fu-Wen Kuo, Sujune Tsai, Chiahsin Lin Sci Rep. 2017; 7: 39396. Published online 2017 Jan 9. doi: 10.1038/srep39396

PMCID: PMC5220285


Characteristics of extended-spectrum β-lactamase–producing Escherichia coli isolated from fecal samples of piglets with diarrhea in central and southern Taiwan in 2015

Wan-Chen Lee, Kuang-Sheng Yeh BMC Vet Res. 2016; 13: 66. Published online 2017 Mar 1. doi: 10.1186/s12917-017-0986-7

PMCID: PMC53334408

Odoo - Sample 3 for three columns

FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng DNA or 1 ng total RNA

  • A safer alternative to EtBr 

  • Suitable to blue or UV light 


Odoo - Sample 3 for three columns

FluoroStain™ DNA Fluorescent Staining Dye

  • Excellent for post staining

  • Sensitivity up to 0.04 ng DNA

  • A safe alternative to EtBr

  • Suitable for blue or UV light

Odoo - Sample 3 for three columns

FluoroDye™ DNA Fluorescent Loading Dye

  • Excellent for premix with DNA sample 

  • Sensitivity up to 0.14 ng DNA

  • Safety dye

  • Convenience - monitor the electrophoresis in real-time

Odoo - Sample 1 for three columns

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

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Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.  

Odoo - Sample 3 for three columns

Colony PCR

Analyze colonies with PCR master mix to save preparation time.