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  3. [TP1000] ExcelTaq™ Taq DNA Polymerase, 5 U/μl, 500 U

[TP1000] ExcelTaq™ Taq DNA Polymerase, 5 U/μl, 500 U

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Description

ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.


Features

  • 5'→3' DNA polymerase activity

  • 5'→3' exonuclease activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3’-dA overhangs

  • Thermo-stable – half-life  lasts for more than 40 min at 95°C


Applications 

  • Routine PCR 

  • Amplification of DNA fragments up to 8 kb

  • Generation of PCR products for TA cloning

  • DNA labeling


Storage

-20°C for 24 months

Odoo - Sample 1 for three columns

Elongation capability

ExcelTaq™ Taq DNA Polymerase can amplify PCR products from λDNA up to 15 kb (M: DM3100).  

Odoo - Sample 2 for three columns

Sensitivity

ExcelTaq™ Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM3100).   

 

Contents

Component

Volume    

Taq DNA Polymerase (5 U/μl)

100 μl      

10X Taq  Buffer

1 ml x 2


Storage Buffer

20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol


10X Taq buffer

200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100


Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 


Storage

-20°C for 24 months 

 

Recommended PCR Condition

 

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

10X Taq Buffer

5 µl

dNTPs

0.2 mM (each)

Taq DNA Polymerase

0.25 µl (1.25U)

ddH2O

to 50 µl

Total volume

50 µl

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C*

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.


Periodontitis promotes the expression of gingival transmembrane serine protease 2 (TMPRSS2),a priming protease for severe acute respiratory syndrome coronavirus 2

(SARS-CoV-2).                           

Ohnishi T, Nakamura T, Shima K, Noguchi K, Chiba N, Matsuguchi T.           

J Oral Biosci. 2022 Jun;64(2):229-236. doi: 10.1016/j.job.2022.04.004. Epub 2022 Apr 25.

PMCID: PMC9035663


Repurposing existing drugs: identification of SARS-CoV-2 3C-like protease inhibitors.

Chiou WC, Hsu MS, Chen YT, Yang JM, Tsay YG, Huang HC, Huang C.       

J Enzyme Inhib Med Chem. 2021 Dec;36(1):147-153. doi: 10.1080/14756366.2020.1850710.

PMCID: PMC7808739


The inhibitory effects of PGG and EGCG against the SARS-CoV-2 3C-like protease

Wei-Chung Chiou, Jui-Chieh Chen, Yun-Ti Chen, Jinn-Moon Yang, Lih-Hwa Hwang, Yi-Shuan Lyu, Hsin-Yi Yang, Cheng Huang, Biochem Biophys Res Commun.
2022 Feb 5;591:130-136. doi: 10.1016/j.bbrc.2020.12.106. Epub 2021 Jan 6.
PMCID: PMC7787066


Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation.
Chen TJ, Wang HJ, Liu JS, Cheng HH, Hsu SC, Wu MC, Lu CH, Wu YF, Wu JW, Liu YY, Kung HJ, Wang WC.
Commun Biol. 2019 Mar 15;2:105. doi: 10.1038/s42003-019-0343-4. eCollection 2019.
PMCID: PMC6420622 


Odoo - Sample 1 for three columns

[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

Odoo - Sample 3 for three columns

[TP5000] ExcelTaq™ Hot Start II DNA Polymerase

  • Aptamer-based hot start PCR

  • Reversible enzyme inactivation

  • Omits extra enzyme activation step

  • Convenient for room temperature PCR set-up

  • High yield and specificity of target amplicons

  • Wide range of amplicon length (up to 10 kb)

  • High sensitivity (as low as 1 fg of plasmid)

Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High Stability

  • Fast Hot Start

  • High Sensitivity

  • Low Background / High Specificity 

  • Suitable for Fast Program 

  • Smart Blue Contrast Dye 

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR