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  3. [TP1000] ExcelTaq™ Taq DNA Polymerase, 5 U/μl, 500 U

[TP1000] ExcelTaq™ Taq DNA Polymerase, 5 U/μl, 500 U

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Description

ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.


Features

  • 5'→3' DNA polymerase activity

  • 5'→3' exonuclease activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3’-dA overhangs

  • Thermo-stable – half-life  lasts for more than 40 min at 95°C


Applications 

  • Routine PCR 

  • Amplification of DNA fragments up to 8 kb

  • Generation of PCR products for TA cloning

  • DNA labeling


Storage

-20°C for 24 months

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Elongation capability

ExcelTaq™ Taq DNA Polymerase can amplify PCR products from λDNA up to 15 kb (M: DM3100).  

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Sensitivity

ExcelTaq™ Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM3100).   

 

Contents

Component

Volume    

Taq DNA Polymerase (5 U/μl)

100 μl      

10X Taq  Buffer

1 ml x 2


Storage Buffer

20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol


10X Taq buffer

200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100


Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 


Storage

-20°C for 24 months 

 

Recommended PCR Condition

 

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

10X Taq Buffer

5 µl

dNTPs

0.2 mM (each)

Taq DNA Polymerase

0.25 µl (1.25U)

ddH2O

to 50 µl

Total volume

50 µl

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C*

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.


 The inhibitory effects of PGG and EGCG against the SARS-CoV-2 3C-like protease

Wei-Chung Chiou, Jui-Chieh Chen, Yun-Ti Chen, Jinn-Moon Yang, Lih-Hwa Hwang, Yi-Shuan Lyu, Hsin-Yi Yang, Cheng Huang, Biochem Biophys Res Commun.
2022 Feb 5;591:130-136. doi: 10.1016/j.bbrc.2020.12.106. Epub 2021 Jan 6.
 
PMCID: PMC7787066


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[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

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[TP5000] ExcelTaq™ Hot Start II DNA Polymerase

  • Aptamer-based hot start PCR

  • Reversible enzyme inactivation

  • Omits extra enzyme activation step

  • Convenient for room temperature PCR set-up

  • High yield and specificity of target amplicons

  • Wide range of amplicon length (up to 10 kb)

  • High sensitivity (as low as 1 fg of plasmid)

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[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High Stability

  • Fast Hot Start

  • High Sensitivity

  • Low Background / High Specificity 

  • Suitable for Fast Program 

  • Smart Blue Contrast Dye 

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[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR