[TK1000] ExcelTaq™ Klen-Taq DNA Polymerase, (5 U/μl, 500 U)

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Description 

ExcelTaq™ Klen-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. The ExcelTaq Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.


Features

  • 5'→3' DNA polymerase activity

  • 3'→5' exonuclease activity (proofreading)

  • Thermo-stable up to 98°C during PCR denaturing step

  • 4× fidelity as compared to Taq DNA polymerase

  • Robust PCR performance, resistance to variance in PCR condition 


Storage

-20°C for 24 months 

Odoo - Sample 1 for three columns

Elongation capability

ExcelTaq™ Klen-Taq DNA Polymerase can amplify PCR products from λDNA up to 12 kb (M: DM5100).  

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Sensitivity

ExcelTaq™ Klen-Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM2100).   

 

Contents

Component

Volume

Klen-Taq DNA Polymerase (5 U/μl)

100 μl

10X Klen Buffer

1.2 ml



ExcelTaq™ Klen-Taq DNA Polymerase mixture

Klen-Taq DNA polymerase             5 U/ µl 

A proofreading DNA polymerase   Trace 


Storage Buffer

40 mM Tris-HCl (pH 7.5), 50 mM KCl, 25 mM (NH4)2SO4, 0.1 mM EDTA, 5.0 mM 2-mercaptoethanol, 50% (v/v) glycerol


10X Klen Buffer

400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin


Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 


Storage

-20°C for 24 months 

 


 

Recommended PCR Condition

 

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

10X Klen Buffer

5 µl

dNTPs

0.2 mM (each)

Klen-Taq enzyme

0.5 µl (2.5 U)

ddH2O

to 50 µl

Total volume

50 µl

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C

30 sec

Extension

68°C

30 sec/kb

Final extension

68°C

1 min

1




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[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

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[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

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[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR