[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)

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Description 

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products. 


Additional Format

The SMO-HiFi™ DNA Polymerase is also available in a master mix format. The SMO-HiFi™ 2X PCR Master Mix (Cat. No. TF1100) is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of primers and template. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors


Features

  • 5'→3' DNA polymerase activity

  • 3'→5' exonuclease activity (proofreading)

  • High reaction rate (up to 1 kb/10 second)

  • High fidelity, 70 times higher than Taq DNA polymerase 

  • Blunt end amplicon

  • Thermo-stable-half life is more than 10 hrs at 95°C 


Storage

[TF1000] SMO-HiFi™ DNA Polymerase

-20°C for 24 months

[TF1100] SMO-HiFi™ 2X PCR Master Mix

4°C for 6 months

Odoo - Sample 1 for three columns

Elongation capability

SMO-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 12 kb in length (M: DM5100). 

Odoo - Sample 2 for three columns

Sensitivity

SMO-HiFi™ DNA Polymerase can amplify PCR products from as little as 1 fg of template DNA (M: DM2000). 

  

[TF1000] SMO-HiFi™ DNA Polymerase

Contents

Component

Volume

SMO-HiFi™ DNA Polymerase (1 U/μl)

100 μl

10X HiFi™ Buffer

600 μl

25 mM MgSO4

500 μl

dNTPs Mix (2 mM each)

600 μl

DMSO

600 μl


Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol


Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 


Storage

-20°C for 24 months 


[TF1100] SMO-HiFi™ 2X PCR Master Mix

Contents

Component

Volume

SMO-HiFi™ 2X PCR Master Mix

1.25 ml

6X DNA Loading Dye (Blue)

1 ml


Storage

4°C for 6 months 


 

[TF1000] SMO-HiFi™ DNA Polymerase

Manual

Manual_TF1000_SMO-HiFi™ DNA Polymerase

MSDS

MSDS_TF1000


[TF1100] SMO-HiFi™ 2X PCR Master Mix

Manual

Manual_TF1100_SMO-HiFi™ 2X Master Mix

MSDS

MSDS_TF1100


SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000) contain different high fidelity DNA polymerases. SMO-HiFi™ (TF1000) and G-HiFi™(TF3000) DNA polymerases are suitable for GC-rich templates that are difficult to amplify.  Moreover G-HiFi™  (TF3000) is blended with elongation enhancer to perform quick and accurate PCR. 

 


 

Recommended PCR Condition 

[TF1000] SMO-HiFi™ DNA Polymerase

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

10X HiFi Buffer

5 µl

MgSO4 (25 mM)

2 μl

dNTPs (2 mM each)

5 µl

SMO-HiFi DNA Polymerase

1 µl (1 unit)

DMSO

5 µl

H2O

to 50 µl

Total volume

50 µl

 

 [TF1100] SMO-HiFi™ 2X PCR Master Mix

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

SMO-HiFi™ 2X PCR Master Mix

25 µl

H2O

to 50 µl

Total volume

50 µl


Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C

30 sec

Extension

68°C

30 sec/kb

Final extension

68°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties. 

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

Odoo - Sample 3 for three columns

Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.

Odoo - Sample 3 for three columns

Colony PCR

Analyze colonies with PCR master mix to save preparation time.