[RP1400] ExcelRT™ Reverse Transcription Kit II, 100 Rxn

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Description 

ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR. 


Features

  • Contains all components for reverse transcription 

  • High yield 

  • Thermostable, up to 50°C 

  • Reduced RNase H ribonuclease activity 

  • Suitable for real-time PCR 


Application

  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C. 


Storage

-20°C for 24 months


 

Contents

Component

Volume

RTase/RI Enzyme Mix 

100 μl

5X RT Buffer (DTT/dNTPs)

500 μl

Oligo (dT)/Random Primer Mix

100 μl

DEPC-Treated H2O

2 x 1 ml


RTase/RI Enzyme Mix

ExcelRT™ Reverse Transcriptase (200 U/μl), RNAok™ RNase Inhibitor (20 U/μl), 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, and 50% (v/v) glycerol


5X RT buffer (DTT/dNTPs)

250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT and 2.5 mM dNTPs (each)


Oligo (dT)/Random Primer Mix

50 μM oligo (dT)20 and 100 μM random hexamers

 



Evolutionary dynamics of SARS-CoV-2 circulating in Yogyakarta and Central Java, Indonesia: sequence analysis covering furin cleavage site (FCS) region of the spike protein

Nastiti Wijayanti, Faris Muhammad Gazali, Endah Supriyati, Mohamad Saifudin Hakim, Eggi Arguni, Marselinus Edwin Widyanto Daniwijaya, Titik Nuryastuti, Matin Nuhamunada, Rahma Nabilla, Sofia Mubarika Haryana, Tri Wibawa, Int Microbiol. 2022 Feb 14;1-10. doi: 10.1007/s10123-022-00239-8.

PMCID: PMC8853438


Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis  

Faris Muhammad Gazali, Matin Nuhamunada, Rahma Nabilla, Endah Supriyati, Mohamad Saifudin Hakim, Eggi Arguni, Edwin Widyanto Daniwijaya, Titik Nuryastuti, Sofia Mubarika Haryana, Tri Wibawa, Nastiti Wijayanti, Heliyon. 2021 Sep;7(9):e07936. doi: 10.1016/j.heliyon.2021.e07936. Epub 2021 Sep 6.

PMCID: PMC8420086


Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

  • High sensitivity

  • High specificity

  • With ROX reference dye

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR

Odoo - Sample 1 for three columns

[RQ2110] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX)

  • High yield 

  • Reverse transcription at wide temperature range (42-60°C) 

  • High specificity

  • Suitable for fast cycle program 

  • With ROX reference dye