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  2. [RP1400] ExcelRT™ Reverse Transcription Kit II, 100 RXN

[RP1400] ExcelRT™ Reverse Transcription Kit II, 100 RXN

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Description 

ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR. 


Features

  • Contains all components for reverse transcription 

  • High yield 

  • Thermostable, up to 50°C 

  • Reduced RNase H ribonuclease activity 

  • Suitable for real-time PCR 


Application

  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C. 


Storage

-20°C for 24 months


 

Contents

Component

Volume    

RTase/RI Enzyme Mix 

100 μl      

5X RT Buffer (DTT/dNTPs)

500 μl      

Oligo (dT)/Random Primer Mix

100 μl      

DEPC-Treated H2O

1 ml x 2


RTase/RI Enzyme Mix

ExcelRT™ Reverse Transcriptase (200 U/μl), RNAok™ RNase Inhibitor (20 U/μl), 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, and 50% (v/v) glycerol


5X RT buffer (DTT/dNTPs)

250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT and 2.5 mM dNTPs (each)


Oligo (dT)/Random Primer Mix

50 μM oligo (dT)20 and 100 μM random hexamers

ExcelRT™ Reverse Transcription Kits contain all components to synthesize high quality first strand cDNA. The components in ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400) are the same. 

The components in RP1300 are separated. This make RP1300 more flexible to fit different experimental requirements. 

Selected components in RP1400 are pre-mixed. This make RP1400 more convenient to use. 

 



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Odoo - Sample 2 for three columns

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  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

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  • High specificity

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Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

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Odoo - Sample 1 for three columns

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