[RP1400] ExcelRT™ Reverse Transcription Kit II, 100 Rxn

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Description

ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR.

Features

Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR

Application

Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.

Storage

-20°C for 24 months

Component

Volume

RTase/RI Enzyme Mix

100 μl

5X RT Buffer (DTT/dNTPs)

500 μl

Oligo (dT)/Random Primer Mix

100 μl

DEPC-Treated H₂O

1 ml x 2

RTase/RI Enzyme Mix

ExcelRT™ Reverse Transcriptase (200 U/μl), RNAok™ RNase Inhibitor (20 U/μl), 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, and 50% (v/v) glycerol

5X RT buffer (DTT/dNTPs)

250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl₂, 50 mM DTT and 2.5 mM dNTPs (each)

Oligo (dT)/Random Primer Mix

50 μM oligo (dT)₂₀ and 100 μM random hexamers

Manual

Manual_RP1400_ExcelRT™ Reverse Transcription Kit II (2023 ver 2.3.0)

SDS

SDS_RP1400

What is the difference between ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400)?

ExcelRT™ Reverse Transcription Kits contain all components to synthesize high quality first strand cDNA. The components in ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400) are the same.

The components in RP1300 are separated. This make RP1300 more flexible to fit different experimental requirements.

Selected components in RP1400 are pre-mixed. This make RP1400 more convenient to use.

Identification of co-regulated genes associated with doxorubicin resistance in the MCF-7/ADR cancer cell line

Ali Miri, Javad Gharechahi, Iman Samiei Mosleh, Kazem Sharifi, Vahid Jajarmi. Front Oncol. 2023; 13: 1135836. Published online 2023 Jun 15. doi: 10.3389/fonc.2023.1135836

PMCID: PMC10311417

Effects of Dietary Lactic Acid Supplementation on the Activity of Digestive and Antioxidant Enzymes, Gene Expressions, and Bacterial Communities in the Intestine of Common Carp, Cyprinus carpio

Seyyed Morteza Hoseini, Morteza Yousefi, Alireza Afzali-Kordmahalleh, Esmaeil Pagheh, Ali Taheri Mirghaed

Animals (Basel) 2023 Jun; 13(12): 1934. Published online 2023 Jun 9. doi: 10.3390/ani13121934

PMCID: PMC10295004

The high mutation rate at the D614G hotspot-furin cleavage site region increases the priming efficiency of the Spike protein by furin protease: analysis of Indonesian SARS-CoV-2 G614 variants obtained during the early COVID-19 pandemic

Faris Muhammad Gazali, Nastiti Wijayanti, Mohamad Saifudin Hakim, Endah Supriyati, Eggi Arguni, Marselinus Edwin Widyanto Daniwijaya, Titik Nuryastuti, Matin Nuhamunada, Rahma Nabilla, Sofia Mubarika Haryana, Tri Wibawa. Virusdisease. 2023 May 31 : 1–10. doi: 10.1007/s13337-023-00827-w [Epub ahead of print]

PMCID: PMC10231289

Amino acid profile changes during enrichment of spheroid cells with cancer stem cell properties in MCF‐7 and MDA‐MB‐231 cell lines

Zahra Ghanbari Movahed, Maryam M. Matin, Kamran Mansouri, Sajjad Sisakhtnezhad.

Cancer Rep (Hoboken) 2023 May; 6(5): e1809. Published online 2023 Apr 24. doi: 10.1002/cnr2.1809

PMCID: PMC10172158

YUSTINA CAROLINA FEBRIANTI SALSINHA, SITI NURBAITI, ALFINO SEBASTIAN,DIDIK INDRADEWA, YEKTI ASIH PURWESTRI, DIAH RACHMAWATI, BIODIVERSITAS 2022 July Volume 23, Number 7, Pages: 3573-3583

Evolutionary dynamics of SARS-CoV-2 circulating in Yogyakarta and Central Java, Indonesia: sequence analysis covering furin cleavage site (FCS) region of the spike protein

Nastiti Wijayanti, Faris Muhammad Gazali, Endah Supriyati, Mohamad Saifudin Hakim, Eggi Arguni, Marselinus Edwin Widyanto Daniwijaya, Titik Nuryastuti, Matin Nuhamunada, Rahma Nabilla, Sofia Mubarika Haryana, Tri Wibawa, Int Microbiol. 2022 Feb 14;1-10. doi: 10.1007/s10123-022-00239-8.

PMCID: PMC8853438

Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis

Faris Muhammad Gazali, Matin Nuhamunada, Rahma Nabilla, Endah Supriyati, Mohamad Saifudin Hakim, Eggi Arguni, Edwin Widyanto Daniwijaya, Titik Nuryastuti, Sofia Mubarika Haryana, Tri Wibawa, Nastiti Wijayanti, Heliyon. 2021 Sep;7(9):e07936. doi: 10.1016/j.heliyon.2021.e07936. Epub 2021 Sep 6.

PMCID: PMC8420086

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