![[RP1400] ExcelRT™ Reverse Transcription Kit II, 100 Rxn](/web/image/product.template/275/image?unique=22d1106)
Description
ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR.
Features
Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR
Application
Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.
Storage
-20°C for 24 months
Contents
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RTase/RI Enzyme Mix
ExcelRT™ Reverse Transcriptase (200 U/μl), RNAok™ RNase Inhibitor (20 U/μl), 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, and 50% (v/v) glycerol
5X RT buffer (DTT/dNTPs)
250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT and 2.5 mM dNTPs (each)
Oligo (dT)/Random Primer Mix
50 μM oligo (dT)20 and 100 μM random hexamers
What is the difference between ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400)?
ExcelRT™ Reverse Transcription Kits contain all components to synthesize high quality first strand cDNA. The components in ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400) are the same.
The components in RP1300 are separated. This make RP1300 more flexible to fit different experimental requirements.
Selected components in RP1400 are pre-mixed. This make RP1400 more convenient to use.
YUSTINA CAROLINA FEBRIANTI SALSINHA, SITI NURBAITI, ALFINO SEBASTIAN,DIDIK INDRADEWA, YEKTI ASIH PURWESTRI, DIAH RACHMAWATI, BIODIVERSITAS 2022 July Volume 23, Number 7, Pages: 3573-3583
Nastiti Wijayanti, Faris Muhammad Gazali, Endah Supriyati, Mohamad Saifudin Hakim, Eggi Arguni, Marselinus Edwin Widyanto Daniwijaya, Titik Nuryastuti, Matin Nuhamunada, Rahma Nabilla, Sofia Mubarika Haryana, Tri Wibawa, Int Microbiol. 2022 Feb 14;1-10. doi: 10.1007/s10123-022-00239-8.
PMCID: PMC8853438
Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis
Faris Muhammad Gazali, Matin Nuhamunada, Rahma Nabilla, Endah Supriyati, Mohamad Saifudin Hakim, Eggi Arguni, Edwin Widyanto Daniwijaya, Titik Nuryastuti, Sofia Mubarika Haryana, Tri Wibawa, Nastiti Wijayanti, Heliyon. 2021 Sep;7(9):e07936. doi: 10.1016/j.heliyon.2021.e07936. Epub 2021 Sep 6.
PMCID: PMC8420086
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High sensitivity and signal intensity
Compatible with fast PCR program
With smart blue contrast dye as a visual aid for reaction setup
Low background
High stability
[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
High sensitivity
High specificity
With ROX reference dye
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix
5’→3’ DNA polymerase activity
No detectable 3'→5' exonuclease (proofreading) activity
Generates PCR products with 3'-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
[RQ2110] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX)
High yield
Reverse transcription at wide temperature range (42-60°C)
High specificity
Suitable for fast cycle program
With ROX reference dye