[RP1400] ExcelRT™ Reverse Transcription Kit II, 100 Rxn

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Description 

ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR. 


Features

  • Contains all components for reverse transcription 

  • High yield 

  • Thermostable, up to 50°C 

  • Reduced RNase H ribonuclease activity 

  • Suitable for real-time PCR 


Application

  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C. 

 

Contents

Component

Volume

RTase/RI Enzyme Mix 

100 μl

5X RT Buffer (DTT/dNTPs)

500 μl

Oligo (dT)/Random Primer Mix

100 μl

DEPC-Treated H2O

2 x 1 ml


RTase/RI Enzyme Mix

ExcelRT™ Reverse Transcriptase (200 U/μl), RNAok™ RNase Inhibitor (20 U/μl), 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, and 50% (v/v) glycerol


5X RT buffer (DTT/dNTPs)

250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT and 2.5 mM dNTPs (each)


Oligo (dT)/Random Primer Mix

50 μM oligo (dT)20 and 100 μM random hexamers

 


Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

  • High sensitivity

  • High specificity

  • With ROX reference dye

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR

Odoo - Sample 1 for three columns

[RQ2110] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX)

  • High yield 

  • Reverse transcription at wide temperature range (42-60°C) 

  • High specificity

  • Suitable for fast cycle program 

  • With ROX reference dye