[RP1300] ExcelRT™ Reverse Transcription Kit, 100 Rxn

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Description 

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 


Features

  • Contains all components for reverse transcription 

  • High yield 

  • Thermostable, up to 50°C, during first strand synthesis 

  • High processivity, generating cDNA up to 8 kb 

  • Reduced RNase H ribonuclease activity 


Application

  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.


Storage

-20°C for 24 months 


Odoo - Sample 1 for three columns

High yield

 

Odoo - Sample 3 for three columns

High sensitivity


Odoo - Sample 2 for three columns

Thermostable, up to 50°C, during first strand synthesis

 

 

Contents

Component

Volume

ExcelRT™ Reverse Transcriptase (200 U/μl) 

100 μl

RNase Inhibitor (20 U/μl)

100 μl

5X RT Buffer (DTT)

500 μl

dNTP Mix (10 mM each)

200 μl

Oligo (dT)20 (50 μM)

100 μl

Random Hexamers (100 μM)

100 μl

DEPC-Treated H2O

2 x 1 ml


Storage Buffer

Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol

RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol


5X RT buffer (DTT)

250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2 and 50 mM DTT


Storage

-20°C for 24 months 

 



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Mohammadarian Akbari, Reyhane Eghtedarian, Bashdar Mahmud Hussen, Solat Eslami, Mohammad Taheri, Seyedeh Morvarid Neishabouri, Soudeh Ghafouri-Fard, Front Mol Neurosci. 2022 Jul 4;15:939224. doi: 10.3389/fnmol.2022.939224. eCollection 2022.

PMCID: PMC9289514


Anti-inflammatory function of apolipoprotein B-depleted plasma is impaired in non-alcoholic fatty liver disease

Negar Sarmadi, Hossein Poustchi, Fatemeh Ali Yari, Amir Reza Radmard, Sara Karami, Abbas Pakdel, Parisa Shabani, Ali Khaleghian, PLoS One. 2022 Apr 12;17(4):e0266227. doi: 10.1371/journal.pone.0266227. eCollection 2022.

PMCID: PMC9004768


Expression analysis of IFNAR1 and TYK2 transcripts in COVID-19 patients

Mohammadarian Akbari, Mehdi Akhavan-Bahabadi, Navid Shafigh, Afshin Taheriazam, Bashdar Mahmud Hussen, Arezou Sayad, Mohadeseh Fathi, Mohammad Taheri, Soudeh Ghafouri-Fard, Mohammad Fathi, Cytokine. 2022 May;153:155849. doi: 10.1016/j.cyto.2022.155849. Epub 2022 Mar 4.

PMCID: PMC8894869


Focal adhesion kinase-related pathways may be suppressed by metformin in vascular smooth muscle cells in high glucose conditions

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PMCID: 35633523


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Davide Vacca, Antonino Fiannaca, Fabio Tramuto, Valeria Cancila, Laura La Paglia, Walter Mazzucco, Alessandro Gulino, Massimo La Rosa, Carmelo Massimo Maida, Gaia Morello, Beatrice Belmonte, Alessandra Casuccio, Rosario Maugeri, Gerardo Iacopino, Carmela Rita Balistreri, Francesco Vitale, Claudio Tripodo, Alfonso Urso, Life (Basel). 2022 Jan 4;12(1):69. doi: 10.3390/life12010069.

PMCID: PMC8778588


Lycopene improves testicular damage and sperm quality in experimentally induced varicocele: Relationship with apoptosis, hypoxia, and hyperthermia

Atefeh Babaei, Reza Asadpour, Kamran Mansouri, Adel Sabrivand, Siamak Kazemi-Darabadi, Food Science & Nutrition March 2022. doi:10.1002/fsn3.2762 


Expression analysis of IFNAR1 and TYK2 transcripts in COVID-19 patients

Mohammadarian Akbari, Mehdi Akhavan-Bahabadi, Navid Shafigh, Afshin Taheriazam, Bashdar Mahmud Hussen, Arezou Sayad, Mohadeseh Fathi, Mohammad Taheri, Soudeh Ghafouri-Fard, Mohammad Fathi, Cytokine. 2022 Mar 4;153:155849. doi: 10.1016/j.cyto.2022.155849. 

PMCID: PMC8894869


Up-regulation of FOXN3-AS1 in invasive ductal carcinoma of breast cancer patients 

Samira Molaei Ramshe 1, Hamid Ghaedi 1, Mir Davood Omrani 2, Lobat Geranpayeh 3, Behnam Alipour 4, Soudeh Ghafouri-Fard, Heliyon

. 2021 Oct 14;7(10):e08179. doi: 10.1016/j.heliyon.2021.e08179. eCollection 2021 Oct.

PMCID: PMC8526775


Expression Analysis of Long Non-Coding RNAs Related With FOXM1, GATA3, FOXA1 and ESR1 in Breast Tissues

Bita Hassani,  Mohammad Taheri,  Yazdan Asgari,  Ali Zekri,  Ali Sattari,  Soudeh Ghafouri-Fard, * and Farkhondeh Pouresmaeili , Front Oncol. 2021; 11: 671418. Published online 2021 May 19. doi: 10.3389/fonc.2021.671418

PMCID: PMC8171254


Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis via Suppression of C-C Chemokine Receptor Type 7 Expression

Pelin Kaya, Sang R. Lee, Young Ho Lee, Sun Woo Kwon, Hyun Yang, Hye Won Lee, and Eui-Ju Hong1, Nutrients. 2019 Feb; 11(2): 410. Published online 2019 Feb 15. doi: 10.3390/nu11020410

PMCID: PMC6412318


Odoo - Sample 1 for three columns

[RP1400] ExcelRT™ Reverse Transcription Kit II

  • Contains all components for reverse transcription

  • High yield 

  • Thermostable, up to 50°C 
    Reduced RNase H ribonuclease activity 

  • Suitable for real-time PCR 

Odoo - Sample 3 for three columns

[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR