[RP1300] ExcelRT™ Reverse Transcription Kit, 100 Rxn

Description

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.

Features

Contains all components for reverse transcription
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity

Application

Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.

Storage

-20°C for 24 months

High yield

High sensitivity

Thermostable, up to 50°C, during first strand synthesis

What is the difference between ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400)?

ExcelRT™ Reverse Transcription Kits contain all components to synthesize high quality first strand cDNA. The components in ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400) are the same.

The components in RP1300 are separated. This make RP1300 more flexible to fit different experimental requirements.

Selected components in RP1400 are pre-mixed. This make RP1400 more convenient to use.

ExcelRT™ Reverse Transcription Kit (RP1300) contains Random hexamers and Oligo(dT)₂₀, how do I choose?

You can choose oligo d(T)₂₀, Random hexamers, or both depending on the RNA template and the downstream applications.

Oligo (dT)20 anneals to poly(A) tails of eukaryotic mRNAs. It is suitable for cDNA libraries construction from eukaryotic mRNAs, full- length cDNA cloning, and 3′ rapid amplification of cDNA ends (3′ RACE). It is not suitable for cDNA synthesis from degraded RNA or RNAs that lack poly(A) tails, such as prokaryotic RNAs and microRNAs.

Random hexamers anneals to any RNA species, including poly(A) tails of eukaryotic mRNAs, degraded RNA, prokaryotic RNA, and RNAs that lack poly(A) tails (rRNA, tRNA, non-coding RNAs, small RNAs, and etc.) It is suitable for gene expression analysis. It is not suitable for full- length cDNA synthesis.

Combine oligo (dT)₂₀ and Random hexamers anneal to any RNA species. Oligo (dT)₂₀ provides additional coverage of 3’ end of RNA templates. It is suitable for gene expression analysis. It is not suitable for full- length cDNA synthesis.

Heavy metal analysis in of indoor and outdoor dust extracts and cytotoxicity evaluation and inflammation factors on lung, gastric and skin cell lines

Abolfazl Naimabadi, Ahmad Ghasemi, Mahnaz Mohtashami, Jafar Saeidi, Mehdi Bakaeian, Aliakbar Haddad Mashadrizeh, Mohsen Azimi-Nezhad, Ali Akbar Mohammadia

Heliyon. 2022 Dec; 8(12): e12414. Published online 2022 Dec 19. doi: 10.1016/j.heliyon.2022.e12414

PMCID: PMC9803783

Gene expression profile of placentomes and clinical parameters in the cows with retained placenta

Mehdi Moradi, Mahdi Zhandi, Mohsen Sharafi, Arvand Akbari, Mohammad Jafari Atrabi, and Mehdi Totonchi

BMC Genomics. 2022; 23: 760. Published online 2022 Nov 21. doi: 10.1186/ s12864-022-08989-5

PMCID: PMC9677913

Assessment of Expression of Regulatory T Cell Differentiation Genes in Autism Spectrum Disorder

Mohammadarian Akbari, Reyhane Eghtedarian, Bashdar Mahmud Hussen, Solat Eslami, Mohammad Taheri, Seyedeh Morvarid Neishabouri, Soudeh Ghafouri-Fard, Front Mol Neurosci. 2022 Jul 4;15:939224. doi: 10.3389/fnmol.2022.939224. eCollection 2022.

PMCID: PMC9289514

Anti-inflammatory function of apolipoprotein B-depleted plasma is impaired in non-alcoholic fatty liver disease

Negar Sarmadi, Hossein Poustchi, Fatemeh Ali Yari, Amir Reza Radmard, Sara Karami, Abbas Pakdel, Parisa Shabani, Ali Khaleghian, PLoS One. 2022 Apr 12;17(4):e0266227. doi: 10.1371/journal.pone.0266227. eCollection 2022.

PMCID: PMC9004768

Expression analysis of IFNAR1 and TYK2 transcripts in COVID-19 patients

Mohammadarian Akbari, Mehdi Akhavan-Bahabadi, Navid Shafigh, Afshin Taheriazam, Bashdar Mahmud Hussen, Arezou Sayad, Mohadeseh Fathi, Mohammad Taheri, Soudeh Ghafouri-Fard, Mohammad Fathi, Cytokine. 2022 May;153:155849. doi: 10.1016/j.cyto.2022.155849. Epub 2022 Mar 4.

PMCID: PMC8894869

Focal adhesion kinase-related pathways may be suppressed by metformin in vascular smooth muscle cells in high glucose conditions

Ali Akbar Soleimani, Ghasem Ghasmpour, Asghar Mohammadi, Masoomeh Gholizadeh, Borhan Rahimi Abkenar, Mohammad Najafi, Endocrinol Diabetes Metab. 2022 May 28;e351. doi: 10.1002/edm2.351.

PMCID: 35633523

Direct RNA Nanopore Sequencing of SARS-CoV-2 Extracted from Critical Material from Swabs

Davide Vacca, Antonino Fiannaca, Fabio Tramuto, Valeria Cancila, Laura La Paglia, Walter Mazzucco, Alessandro Gulino, Massimo La Rosa, Carmelo Massimo Maida, Gaia Morello, Beatrice Belmonte, Alessandra Casuccio, Rosario Maugeri, Gerardo Iacopino, Carmela Rita Balistreri, Francesco Vitale, Claudio Tripodo, Alfonso Urso, Life (Basel). 2022 Jan 4;12(1):69. doi: 10.3390/life12010069.

PMCID: PMC8778588

Lycopene improves testicular damage and sperm quality in experimentally induced varicocele: Relationship with apoptosis, hypoxia, and hyperthermia

Atefeh Babaei, Reza Asadpour, Kamran Mansouri, Adel Sabrivand, Siamak Kazemi-Darabadi, Food Science & Nutrition March 2022. doi:10.1002/fsn3.2762

Expression analysis of IFNAR1 and TYK2 transcripts in COVID-19 patients

Mohammadarian Akbari, Mehdi Akhavan-Bahabadi, Navid Shafigh, Afshin Taheriazam, Bashdar Mahmud Hussen, Arezou Sayad, Mohadeseh Fathi, Mohammad Taheri, Soudeh Ghafouri-Fard, Mohammad Fathi, Cytokine. 2022 Mar 4;153:155849. doi: 10.1016/j.cyto.2022.155849.

PMCID: PMC8894869

Up-regulation of FOXN3-AS1 in invasive ductal carcinoma of breast cancer patients

Samira Molaei Ramshe 1, Hamid Ghaedi 1, Mir Davood Omrani 2, Lobat Geranpayeh 3, Behnam Alipour 4, Soudeh Ghafouri-Fard, Heliyon

. 2021 Oct 14;7(10):e08179. doi: 10.1016/j.heliyon.2021.e08179. eCollection 2021 Oct.

PMCID: PMC8526775

Expression Analysis of Long Non-Coding RNAs Related With FOXM1, GATA3, FOXA1 and ESR1 in Breast Tissues

Bita Hassani, Mohammad Taheri, Yazdan Asgari, Ali Zekri, Ali Sattari, Soudeh Ghafouri-Fard, * and Farkhondeh Pouresmaeili , Front Oncol. 2021; 11: 671418. Published online 2021 May 19. doi: 10.3389/fonc.2021.671418

PMCID: PMC8171254

Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis via Suppression of C-C Chemokine Receptor Type 7 Expression

Pelin Kaya, Sang R. Lee, Young Ho Lee, Sun Woo Kwon, Hyun Yang, Hye Won Lee, and Eui-Ju Hong1, Nutrients. 2019 Feb; 11(2): 410. Published online 2019 Feb 15. doi: 10.3390/nu11020410

PMCID: PMC6412318

[RP1400] ExcelRT™ Reverse Transcription Kit II

Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR

[TF1000] SMO-HiFi™ DNA Polymerase

5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
High reaction rate—10 seconds/kb
High fidelity— 70 times higher than Taq polymerase
Generates blunt end amplicon
Thermo-stable for more than 10 hrs at 95°C.

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

High sensitivity and signal intensity
Compatible with fast PCR program
With smart blue contrast dye as a visual aid for reaction setup
Low background
High stability

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

5’→3’ DNA polymerase activity
No detectable 3'→5' exonuclease (proofreading) activity
Generates PCR products with 3'-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR