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  3. [RP1000] ExcelRT™ Reverse Transcriptase, 200 U/μl, 20000 U

[RP1000] ExcelRT™ Reverse Transcriptase, 200 U/μl, 20000 U

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Description 

The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.


Features

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity


Application

  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.


Storage

-20°C for 24 months

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High yield

 

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Thermostable, up to 50°C, during first strand synthesis

 

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High sensitivity



Contents

Component

Volume

ExcelRT™ Reverse Transcriptase (200 U/μl) 

100 μl

5X RT Buffer

1 ml

0.1 M DTT   

500 μl


Storage Buffer

20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol 


5X RT buffer

250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl and 15 mM MgCl2


Storage

-20°C for 24 months


Probably, but it depends on the quantity and quality of RNA that individuals use. In most cases, the recommended amount of enzyme delivers the best result. Diluted usage may vary its effectiveness.

 


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[RI1000] RNAok™ RNase Inhibitor

Application

  • RT-PCR

  • cDNA Synthesis 

  • in vitro transcription

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[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity— 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

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[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

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[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR