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  3. [RI1000] RNAok™ RNase Inhibitor, 20 U/μl, 2000 U

[RI1000] RNAok™ RNase Inhibitor, 20 U/μl, 2000 U

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Description 

The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.



Application

  • cDNA synthesis

  • in vitro translation

  • in vitro transcription

  • One-step RT-PCR

  • Separation and identification of specific ribonuclease activities


Storage Buffer

40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT,   0.1 mM EDTA, stabilizer and 50% (v/v) glycerol


Storage

-20°C for 24 months 

Odoo - Sample 1 for three columns

RNAok™ RNase Inhibitor inhibits RNase A activity

Total HeLa RNA (1 µg) was incubated with 1U/µl of RNAok™ RNase Inhibitor and 0.4 ng RNase A for 30 min at 37°C. The result shows that RNAok™ RNase Inhibitor can protect RNA against 0.4 ng RNase A. (M: DM3100) 

 Contents

Component

Volume

Cat. No.

RNAok™ RNase Inhibitor (20 U/µl)

100 µl

RI1000

RNAok™ RNase Inhibitor (20 U/µl)

100 µl x 5

RI1001


Storage

-20°C for 24 months 

 

Usage Recommendation

One microliter (20 units) RNAok™ RNase Inhibitor in total 20 μl of reaction mixture is suggested to protect mRNA and improve total cDNA yield in the first strand cDNA synthesis. The RNAok™ RNase Inhibitor is active at pH of 6.0-9.0. Should NOT be greater than 50℃ to prevent inactivation of RNAok™ RNase Inhibitor.


Odoo - Sample 1 for three columns

[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

Odoo - Sample 3 for three columns

[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity— 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR