[RI1000] RNAok™ RNase Inhibitor, (20 U/μl, 2000 U)

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Description 

RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor that is purified by affinity chromatography from E. coli. This protein inhibits pancreatic-type ribonucleases, RNase A, B, and C by binding strongly to RNases in a noncompetitive mode at a 1:1 ratio. RNAok™ RNase Inhibitor does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Extensive quality control tests ensure RNAok™ RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.


Application

  • RT-PCR

  • cDNA Synthesis 

  • in vitro transcription


Storage Buffer

20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, stabilizer, 50% (v/v) glycerol


Storage

-20°C for 24 months 

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RNAok™ RNase Inhibitor inhibits RNase A activity

Total HeLa RNA (1 µg) was incubated with 1U/µl of RNAok™ RNase Inhibitor and 0.4 ng RNase A for 30 min at 37°C. The result shows that RNAok™ RNase Inhibitor can protect RNA against 0.4 ng RNase A. (M: DM3100) 

 Contents

Component

Volume

Cat. No.

RNAok™ RNase Inhibitor (20 U/µl)

100 µl

RI1000

RNAok™ RNase Inhibitor (20 U/µl)

5 x 100 µl

RI1001


Storage

-20°C for 24 months 

 

Usage Recommendation

Add RNAok™ RNase Inhibitor to transcription, translation, and cDNA synthesis reactions at a final concentration of 1 Unit/µl.


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[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

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[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

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[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

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[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR