[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl

Call For Price




                             

                            Description

                            FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr. 

                            FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.


                            Features:

                            • Excellent for in-gel staining

                            • Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA)

                            • A safer alternative to EtBr

                            • Compatibility: suitable to blue or UV light

                            • Increased cloning efficiency (blue light)

                             

                            Storage

                            Protected from light
                            4°C for 12 months
                            -20°C for 24 months


                            Odoo - Sample 1 for three columns

                            Sensitivity of FluoroVue™

                            The FluoroVue™ Nucleic Acid Gel Stain (NS1000) shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. 

                            Odoo - Sample 2 for three columns

                            Excitation and emission spectrum of FluoroVue™ 

                            FluoroVue™ Nucleic Acid Gel Stain (NS1000) has a fluorescent excitation maxima of ~250 and ~482 nm, and an emission maximum of ~509 nm. Therefore, it can replace EtBr without the need for changing existing lab imaging systems. 

                            Odoo - Sample 3 for three columns

                            Non-mutagenicity of FluoroVue™

                            FluoroVue™ Nucleic Acid Gel Stain (NS1000) is proofed for their safety (non-mutagenicity) using Ames test. However, it must be noted that since solvent may penetrate the skin, it is recommended that users wear gloves when using the fluorescent dyes.

                             Contents

                            Component

                            Volume

                            Cat. No.

                            FluoroVue™ Nucleic Acid Gel Stain (10,000X)

                            500 μl

                            NS1000

                            FluoroVue™ Nucleic Acid Gel Stain (10,000X)

                            5 x 500 μl

                            NS1001

                             

                            Storage

                            Protected from light
                            4°C for 12 months
                            -20°C for 24 months


                             



                            Revisiting chloroplast genomic landscape and annotation towards comparative chloroplast genomes of Rhamnaceae

                            Kwanjeera Wanichthanarak, Intawat Nookaew, Phongthana Pasookhush, Thidathip Wongsurawat, Piroon Jenjaroenpun, Namkhang Leeratsuwan, Songsak Wattanachaisaereekul, Wonnop Visessanguan, Yongyut Sirivatanauksorn, Narong Nuntasaen, Chutima Kuhakarn, Vichai Reutrakul, Pravech Ajawatanawong, Sakda Khoomrung

                            BMC Plant Biol. 2023 Jan 28;23(1):59. doi: 10.1186/s12870-023-04074-5.

                            PMCID: PMC9883906


                            Difference between Microscopic and PCR Examination Result for Malaria Diagnosis and Treatment Evaluation in Sumba Barat Daya, Indonesia

                            Dwita Anastasia Deo, Elizabeth Henny Herningtyas, Umi Solekhah Intansari, Taufik Mulya Perdana, Elsa Herdiana Murhandarwati, Marsetyawan H N E Soesatyo, Trop Med Infect Dis. 2022 Jul 29;7(8):153. doi: 10.3390/tropicalmed7080153.

                            PMCID: PMC9412636


                            Squash Leaf Curl Virus: Species of Begomovirus as the Cause of Butternut Squash Yield Losses in Indonesia

                            Dewa Gede Wiryangga Selangga, Listihani Listihani, HAYATI Journal of Biosciences, November 2022; 29(6), 806-813. https://doi.org/10.4308/hjb.29.6.806-813


                            Phylogenetic Marker Selection and Protein Sequence Analysis of the ORF5 Gene Product of Grapevine Virus A

                            Mina Rastgou, Vahid Roumi, Emanuela Noris, Slavica Matić, Sezai Ercisli, Plants (Basel). 2022 Apr 20;11(9):1118. doi: 10.3390/plants11091118.

                            PMCID: PMC9104223 


                            Teaching during COVID-19 pandemic in practical laboratory classes of applied biochemistry and pharmacology: A validated fast and simple protocol for detection of SARS-CoV-2 Spike sequences

                            Jessica Gasparello, Chiara Papi, Matteo Zurlo, Lucia Carmela Cosenza, Giulia Breveglieri, Cristina Zuccato, Roberto Gambari, Alessia Finotti, PLoS One. 2022 Apr 6;17(4):e0266419. doi: 10.1371/journal.pone.0266419. eCollection 2022.

                            PMCID: PMC8985952


                            Ocular surface toll like receptors in ageing

                            Antonio Di Zazzo, Maria De Piano, Marco Coassin, Tommaso Mori, Bijorn Omar Balzamino, Alessandra Micera, BMC Ophthalmol. 2022 Apr 22;22(1):185. doi: 10.1186/s12886-022-02398-8.

                            PMCID: PMC9027701


                            Distribution and molecular characterization of Squash mosaic virus on cucumber in Gianyar, Bali

                            Listihani, Ni Putu Pandawani, Tri Asmira Damayanti, Mimi Sutrawati, Dewa Gede Wiryangga Selangga, Ketut Ayu Yuliadhi, Trisna Agung Phabiola, & Gusti Ngurah Alit Susanta Wirya, J. Trop. Plant Pests Dis. Vol. 22, No. 1, March 2022: 48 –54

                             

                            Identification of Endosymbiotic Virus in Small Extracellular Vesicles Derived from Trichomonas vaginalis

                            Seow-Chin Ong, Wei-Hung Cheng, Fu-Man Ku, Chih-Yu Tsai, Po-Jung Huang, Chi-Ching Lee 4, Yuan-Ming Yeh, Petr Rada, Ivan Hrdý, Ravi Kumar Narayanasamy, Tamara Smutná, Rose Lin, Hong-Wei Luo, Cheng-Hsun Chiu, Jan Tachezy, Petrus Tang, Genes (Basel). 2022 Mar 17;13(3):531. doi: 10.3390/genes13030531.

                            PMCID: PMC8951798


                            Association Between Retinoid X Receptor Gene Variants and Dyslipidemia Risk in an Iranian Population

                            Farzane Vafaeie, Toba Kazemi, Saeede Khosravi, Ebrahim Miri Moghaddam, Cureus. 2021 Sep 5;13(9):e17730. doi: 10.7759/cureus.17730. eCollection 2021 Sep.

                            PMCID: PMC8491560


                            Genetic and parasitological identification of Trypanosoma evansi infecting cattle in South Sulawesi, Indonesia

                            Agus Setiawan, Wisnu Nurcahyo, Dwi Priyowidodo, Rina Tri Budiati, and Desy Sylvia Ratna Susanti, Vet World. 2021 Jan; 14(1): 113–119. Published online 2021 Jan 15. doi: 10.14202/vetworld.2021.113-119

                            PMCID: PMC7896907


                            PCR analysis for assessment of bacterial bioburden in orthokeratology lens case

                            Jung Lo, Po-Chiung Fang, Chun-Chih Chien, Chang-Chun Hsiao, Shin-Ling Tseng, Yu-Hsuan Lai, and Ming-Tse Kuocorresponding author, Mol Vis. 2016; 22: 1–8. Published online 2016 Jan 14

                            PMCID: PMC4734148


                            Epidemiology and clinical features of viral anterior uveitis in southern Taiwan—diagnosis with polymerase chain reaction

                            Yu-Ting Hsiao, Ming-Tse Kuo, Wei-Yu Chiang, Tsai-Ling Chao, and Hsi-Kung Kuo, BMC Ophthalmol. 2019; 19: 87. Published online 2019 Apr 3. doi: 10.1186/s12886-019-1093-2

                            PMCID: PMC6448235

                             

                            Before opening

                            Warm the vial to an ambient temperature, then vortex and spin down the content of the vial to ensure the solution is homogeneous.


                            Working Reagent Preparation

                            1:10,000 dilution in TAE or TBE buffered agarose 

                            Odoo - Sample 1 for three columns

                            In- gel staining

                            This protocol is highly recommended.

                            1. Prepare TAE or TBE buffered molten agarose solution.

                            40 ml molten agarose solution can cast two mini-gels (5.4 x 5.9 cm) or one landscape-gel (10.9 x 5.9 cm).

                            2. Dilute FluoroVue™ Nucleic Acid Gel Stain 10,000X with the molten gel solution and mix well prior to being poured into the gel. 

                            For example: 4 μl in 40 ml molten agarose solution

                            3. Cool the molten agarose solution until it can be handled by hand and then pour it into gel tray.

                            Casted gels are stable at 4°C for 3 days in dark. After three days the sensitivity will decrease daily. 

                            4. Perform agarose gel electrophoresis (avoid light).

                            The recommended voltage is 4–10 V/cm (distance between anode and cathode). Avoid using high voltage during electrophoresis. High voltage causes excess heat and affects the dye adversely. 

                            During electrophoresis, the staining dye runs toward the cathode, therefore DNA bands with smaller molecular weights may be weaker in intensity due to less staining dye. 

                            5. Visualize or photograph the gel with UV or blue-light illumination (blue-light is recommended).

                            Clean the surface of the illuminator before and after each use with deionized water. Accumulation of fluorescent dyes on the surface will create a high fluorescent background.

                            Video cameras and CCD cameras have a different spectral response compared to the black-and-white print film and therefore may not exhibit the same sensitivity.




                            Odoo - Sample 2 for three columns

                            Staining during electrophoresis

                            The sensitivity of this method is slightly lower than the in-gel staining.

                            1. Prepare agarose gel following your standard protocol.

                            2. Dilute FluoroVue™ Nucleic Acid Gel Stain 10,000 folds into the running buffer and mix well.

                            For example: 30 μl in 300 ml running buffer 

                            3. Perform agarose gel electrophoresis (avoid light).

                            During electrophoresis, the staining dye runs toward the cathode .

                            4. Visualize or photograph the gel with UV or blue-light illumination (blue-light is recommended).

                            Clean the surface of the illuminator before and after each use with deionized water. Accumulation of fluorescent dyes on the surface will create a high fluorescent background.

                            Video cameras and CCD cameras have a different spectral response compared to the black-and-white print film and therefore may not exhibit the same sensitivity.

                            Odoo - Sample 2 for three columns

                            Staining after electrophoresis (post-staining)

                            Post-staining method is recommended for polyacrylamide electrophoresis, due to the longer time required for running PAGE. The sensitivity of this method is lower than the in-gel staining method.

                            1. Performing agarose gel electrophoresis following your standard protocol.

                             2. Dilute FluoroVue™ Nucleic Acid Gel Stain 10,000 folds into the TE, TAE, or TBE buffer and mix well.

                            For example: 10 μl in 100 ml TAE buffer 

                            Use a plastic container. Glass containers are not recommended, as they absorb fluorescent dye in staining solution. 

                            Protect the staining container from light by covering it with aluminium foil, or place it in the dark.

                            The staining solution can be stored for up to one week or more.

                            3. Immerse the gel in a staining solution (1X) and incubate at room temperature for 10 - 30 minutes. (avoid light).

                            Staining time varies with the thickness of the gel and percentage of agarose. If needed, agitate the gel gently at room temperature to shorten staining time. 

                            4. Visualize or photograph the gel with UV or blue-light illumination (blue-light is recommended).

                            Clean the surface of the illuminator before and after each use with deionized water. Accumulation of fluorescent dyes on the surface will create a high fluorescent background.

                            Video cameras and CCD cameras have a different spectral response compared to the black-and-white print film and therefore may not exhibit the same sensitivity.




                            Odoo - Sample 3 for three columns

                            B-BOX™ Blue Light LED Epi-illuminator

                            • 470 nm long wavelength

                            • Improved cloning efficiency 

                            • Compact, light-weight, and portable (less than 1 kg)

                            • Adjustable and removable filter plate allows for gel cutting, visualization, and documentation

                            Odoo - Sample 1 for three columns

                            ExcelBand™ DNA Ladder series

                            • Sharp bands

                            • Quick reference enhanced bands 

                            • Ready-to-use— premixed with loading dye for direct loading

                            • Stable— room temperature storage over 6 months

                            Odoo - Sample 3 for three columns

                            FluoroStain™ DNA Fluorescent Staining Dye

                            • Excellent for post staining

                            • Sensitivity up to 0.04 ng DNA

                            • A safe alternative to EtBr

                            • Suitable for blue or UV light