[DM4100] ExcelBand™ XL 25 kb DNA Ladder, Broad Range (up to 25 kb), 500 μl

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Description 

The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. 


Features

  • Sharp bands

  • Quick reference enhanced bands 

  • Ready-to-use— premixed with loading dye for direct loading

  • Stable— room temperature storage over 6 months


Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. 


Range 

250 ~ 25,000 bp 


Concentration 

51.6 µg/ 500 µl 


Recommended loading volume 

5 µl/ well 


Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months

 

Specification

Cat. No.

DM4100

Series Name

ExcelBand™

Product Size

500 μl

Size Range

250 – 25000 bp

Band Number

14

Tracking Dye

Bromophenol blue 
and Xylene cyanol FF

Enhanced Band

1000 and 3000 bp

DNA fragments with identical in size are indistinguishable on agarose gels, but, on acrylamide gels, even slight differences in DNA sequence can lead to noticeably different migration rates. To provide increased intensity of DNA marker/ladder bands, multiple DNA fragments with identical in size but different in DNA sequences are used. SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are more suitable for DNA PAGE.

 


 

The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971

PMCID: PMC5790263


Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)

Katherine Domb, Danielle Keidar, Beery Yaakov, Vadim Khasdan, Khalil Kashkush BMC Plant Biol. 2017; 17: 175. Published online 2017 Oct 27. doi: 10.1186/s12870-017-1134-z

PMCID: PMC5659041

Odoo - Sample 3 for three columns

FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng DNA or 1 ng total RNA

  • A safer alternative to EtBr 

  • Suitable to blue or UV light 


Odoo - Sample 3 for three columns

FluoroStain™ DNA Fluorescent Staining Dye

  • Excellent for post staining

  • Sensitivity up to 0.04 ng DNA

  • A safe alternative to EtBr

  • Suitable for blue or UV light

Odoo - Sample 3 for three columns

FluoroDye™ DNA Fluorescent Loading Dye

  • Excellent for premix with DNA sample 

  • Sensitivity up to 0.14 ng DNA

  • Safety dye

  • Convenience - monitor the electrophoresis in real-time