[DM2300] ExcelBand™ 100 bp+3K DNA Ladder, 500μl

Description

The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.

Features

Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months

Source

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range

100 ~ 3,000 bp

Concentration

56 µg/ 500 µl

Recommended loading volume

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months
-20°C for 36 months

Heading Sample

Specification

Cat. No.	DM2300
Series Name	ExcelBand™
Product Size	500 μl
Size Range	100 – 3000 bp
Band Number	12
Tracking Dye	Orange G and Xylene cyanol FF
Enhanced Band	500 and 1500 bp

Manual

Manual_DM2300_ExcelBand™ 100 bp+3K DNA Ladder (2023 ver. 2.4.0)

SDS

SDS_DM2300

Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?

Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.

Are the SMOBIO's DNA markers/ladders compatible to radio-labeling (for example, label DNA with T4 Polynucleotide Kinase)?

We do not recommend to do the labeling reaction directly.

The reasons are as follows:

Our DNA marker is pre-mixed with loading buffer that contains Tris-HCl, EDTA, and glycerol, may affect radio-labeling.
Our DNA marker is a mixture of PCR products and restriction enzyme digested DNA fragments. Digested dsDNA may still have phospho-group on their 5' end.

To enhance the efficiency of the labeling reaction, here are two steps suggested being done prior to the labeling reaction:

Purify DNA marker by EtOH precipitation or DNA purification kits to remove loading buffer.
Remove phospho-group by using phosphatase, ex CIAP (Calf intestinal alkaline phosphatase)

Are SMOBIO's DNA markers/ladders suitable to use in DNA PAGE?

SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.

Why DNA markers/ladders are resolved two bands at the same size when using high percentage agarose or polyacrylamide gel?

DNA fragments with identical in size are indistinguishable on agarose gels, but, on acrylamide gels, even slight differences in DNA sequence can lead to noticeably different migration rates. To provide increased intensity of DNA marker/ladder bands, multiple DNA fragments with identical in size but different in DNA sequences are used. SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are more suitable for DNA PAGE.

Are SMOBIO's DNA markers/ladders suitable to use in denaturing DNA PAGE?

SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.

SMOBIO's AccuBand™ DNA markers are not at denatured form, therefore, you need to denature DNA ladder by yourself.

Here is the protocol to denature DNA ladder:

Mix 5 μL of DNA Ladder with an equal volume of denaturing solution [95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue, 0.1% (w/v) xylene cyanol].
Incubate at 70°C for 5 minutes.
Electrophorese the sample in a denaturing polyacrylamide/urea gel

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Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light

FluoroStain™ DNA Fluorescent Staining Dye

Excellent for post staining
Sensitivity up to 0.04 ng DNA
A safe alternative to EtBr
Suitable for blue or UV light

FluoroDye™ DNA Fluorescent Loading Dye

Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time