[DM2000] AccuBand™ 100 bp DNA Marker II, 500 μl
Description
AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.
Features
Sharp bands
Suitable for polyacrylamide gel electrophoresis
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 2,000 bp
Concentration
45.5 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
Specification
Cat. No. | DM2000 |
Series Name | AccuBand™ |
Product Size | 500 μl |
Size Range | 100 – 2000 bp |
Band Number | 6 |
Tracking Dye | Orange G and Xylene cyanol FF |
Enhanced Band | 750 bp |
Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?
Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.
Are the SMOBIO's DNA markers/ladders compatible to radio-labeling (for example, label DNA with T4 Polynucleotide Kinase)?
We do not recommend to do the labeling reaction directly.
The reasons are as follows:
Our DNA marker is pre-mixed with loading buffer that contains Tris-HCl, EDTA, and glycerol, may affect radio-labeling.
Our DNA marker is a mixture of PCR products and restriction enzyme digested DNA fragments. Digested dsDNA may still have phospho-group on their 5' end.
To enhance the efficiency of the labeling reaction, here are two steps suggested being done prior to the labeling reaction:
Purify DNA marker by EtOH precipitation or DNA purification kits to remove loading buffer.
Remove phospho-group by using phosphatase, ex CIAP (Calf intestinal alkaline phosphatase)
Are SMOBIO's DNA markers/ladders suitable to use in DNA PAGE?
SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.
Why DNA markers/ladders are resolved two bands at the same size when using high percentage agarose or polyacrylamide gel?
DNA fragments with identical in size are indistinguishable on agarose gels, but, on acrylamide gels, even slight differences in DNA sequence can lead to noticeably different migration rates. To provide increased intensity of DNA marker/ladder bands, multiple DNA fragments with identical in size but different in DNA sequences are used. SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are more suitable for DNA PAGE.
Are SMOBIO's DNA markers/ladders suitable to use in denaturing DNA PAGE?
SMOBIO's AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.
SMOBIO's AccuBand™ DNA markers are not at denatured form, therefore, you need to denature DNA ladder by yourself.
Here is the protocol to denature DNA ladder:
Mix 5 μL of DNA Ladder with an equal volume of denaturing solution [95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue, 0.1% (w/v) xylene cyanol].
Incubate at 70°C for 5 minutes.
Electrophorese the sample in a denaturing polyacrylamide/urea gel
FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
FluoroStain™ DNA Fluorescent Staining Dye
Excellent for post staining
Sensitivity up to 0.04 ng DNA
A safe alternative to EtBr
Suitable for blue or UV light
FluoroDye™ DNA Fluorescent Loading Dye
Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time