[WM1000] YesBlot™ Western Marker I, 250 μl
YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgG-binding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 prestained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)
The YesBlot™ Western Marker I contains recombinant IgG binding proteins, prestained recombinant proteins, glycerol and SDS.
4°C for 3 months
-20°C for 24 months
MW Range (Western Bands)
15 – 200 kDa
MW Range (Pre-stained Bands)
10 – 70 kDa
Band Number (Western Bands)
Band Number (Pre-stained Bands)
Pre-stained Band Color
Principle of YesBlot™ Western Marker I
Manual_WM1000_YesBlot™ Western Marker I (2023 ver. 2.3.0)
Western Marker- Powerful for Western Data Credibility
Migration patterns and approximate MWs (kDa) of pre-stained bands
What is the composition of the YesBlot™ Western Marker I?
YesBlot™ Western Marker I contains ten IgG-binding proteins that binds the primary or secondary IgG antibody used to detect target protein, allowing you to detect the YesBlot™ Western Marker I directly. Addition to IgG-binding proteins, YesBlot™ Western Marker I contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size.
Does YesBlot™ Western Marker I selectively bind to IgG antibodies generated from different host species?
YesBlot™ Western Marker I can bind to IgG antibodies generated from mammalian host species, such as Human, Horse, Cow, Pig, Rat, Mouse, Rabbit, Goat, Sheep, Hamster, Guinea Pig, and etc.
Can YesBlot™ Western Marker I be used for protein quantification?
Since Western blot is a series of signal amplification, different kinds of primary antibodies and secondary antibodies will lead to different signal intensity on the film. We do not recommend using YesBlot™ Western Marker I for quantification purpose.
Will the YesBlot™ Western Marker I be detected using IgM antibodies?
YesBlot™ Western Marker I contains IgG binding proteins, which will not bind to IgM antibodies.
Can YesBlot™ Western Marker I be used with fluorescent-dye conjugated secondary antibody?
YesBlot™ Western Marker I can bind to IgG antibodies labeled with fluorescent dye.
Immunoglobulin Y Specific for SARS-CoV-2 Spike Protein Subunits Effectively Neutralizes SARS-CoV-2 Infectivity and Ameliorates Disease Manifestations In Vivo
Chia-Tsui Yeh, Chia-Ying Lee, Yi-Jung Ho, Sin-An Chen, Liang-Yu Chen, Ping-Cheng Liu, Yuan-Fan Chin, An-Yu Chen, Po-Shiuan Hsieh, Yi-Jen Hung, Cheng-Cheung Chen, Yu-Chie Wang, Guan-Chiun Lee
Biomedicines. 2022 Nov; 10(11): 2774. Published online 2022 Nov 1. doi: 10.3390/biomedicines10112774
ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
Q-PAGE™ Precast Gels
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced gel electrophoresis speed
Better band separation
Stable for shipping at ambient temperature
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems