[RP1100] ExcelRT™ One-Step RT-PCR Kit, 50 RXN

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Description 

The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis 


Features

  • Generates dsDNA directly from RNA samples 

  • High sensitivity and yields 

  • High reproducibility, reduced pipetting errors

  • High throughput 

  • Reduced RNase H ribonuclease activity


Storage

-20°C for 24 months 

 

Contents

Component

Volume

Taq /RT Enzyme Mix

50 μl

2X One-Step Buffer

2 x 750 μl


Storage

-20°C for 24 months 

Odoo - Sample 1 for three columns

High sensitivity and yields 

 

Odoo - Sample 1 for three columns

[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

Odoo - Sample 3 for three columns

[RI1000] RNAok™ RNase Inhibitor

Application

  • RT-PCR

  • cDNA Synthesis 

  • in vitro transcription

Odoo - Sample 2 for three columns

[NS1000] FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng DNA or 1 ng total RNA

  • A safer alternative to EtBr 

  • Suitable to blue or UV light 

Odoo - Sample 3 for three columns

[RQ2110] ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)

  • High yield

  • Reverse transcription at wide temperature range (42-60°C)

  • High specificity 

  • Suitable for fast cycle program

  • With ROX reference dye

 



The Effect of ω3 Fatty Acids Supplementation on Levels of PPARγ and UCP2 Genes Expression, Serum Level of UCP2 Protein, Metabolic Status, and Appetite in Elite male Athletes: Protocol for a Randomized Control Trial  

Sara Moradi, Mohamadreza Alivand, Yaser KhajeBishak, Mohamad AsghariJafarabadi, Maedeh Alipour, Amirhosien Faghfouri, Beitullah Alipour, Int J Surg Protoc. 2021 Aug 19;25(1):184-193. doi: 10.29337/ijsp.161. eCollection 2021.

PMCID: PMC8378073