[PS1000] FluoroStain™ Protein Fluorescent Staining Dye (Red, 1,000X), 1 ml

Description 

The FluoroStain™ Protein Fluorescent Staining Dye (PS1000/PS1001) is a less toxic and more environment-friendly reagent and designed to substitute the Coomassie Blue protein staining method and Silver Stain, offering great sensitivity and ease of operation. Unlike the Coomassie Blue stain, the FluoroStain™ binds to proteins with high specificity and exhibits low affinity to polyacrylamide gel, making destaining an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ Protein Fluorescent Staining Dye is capable of achieving detection levels parallel to silver staining. The FluoroStain™ is also compatible with mass spectrometry analysis, i.e. LC-MS/MS, MALDI-TOF etc., facilitating the use for proteomics analysis. 

The FluoroStain™ Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517nm and emission at 605 nm. The FluoroStain™ is compatible with both ultra violet illumination systems and less harmful blue light illumination system, such as B-BOX™ Blue light epi-illuminator is recommended.

Spectral Characteristics

When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm. In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.

These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.

Storage 

Protected from light 
-20°C for 24 months