[PS1000] FluoroStain™ Protein Fluorescent Staining Dye (Red, 1,000X), 1 ml

Call For Price




 

Description 

The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra (Fig. 2), i.e. LC-MS/MS, MALDI-TOF, etc.


Spectral Characteristics

When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm (Fig. 3). In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.

These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.


Storage 

Protected from light 
-20°C for 24 months 

Odoo - Sample 1 for three columns

Sensitivity of FluoroStain™ 

Comparison of FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) with Silver stain of a 2× serially diluted BSA sample.

Odoo - Sample 2 for three columns

Spectrum of FluoroStain™ 

Excitation and emission spectrum of FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×).

Odoo - Sample 3 for three columns

Compatibility to MASS analysis

Comparison of MALDI-TOF mass spectra of bovine serum albumin (BSA) stained with FluoroStain Protein Fluorescent Staining Dye (A) and with Coomassie Blue (B). BSA proteins are seperated on SDS-PAGE, stained with fluorescent dye or conventional Coomassie Blue, followed by trypsin digestion in gel, and then analyzed by MALDI-TOF. 

Contents

Component

Volume

Cat. No.

FluoroStain™ Protein Fluorescent Staining Dye

1 ml

PS1000

FluoroStain™ Protein Fluorescent Staining Dye

5 x 1 ml

PS1001


 


 

Working Reagent Preparation

1:1000 dilution in 40% ethanol and 2% H3PO4

Suggested volume of staining solution

Gel dimension
(1 mm thick)

Gel volume

1× staining solution

9 cm × 7 cm

≈ 6.5 ml

≈ 100 ml

13 cm × 9 cm

≈ 12 ml

≈ 180 ml

16 cm × 16 cm

≈ 26 ml

≈ 390 ml

26 cm × 23 cm

≈ 60 ml

≈ 900 ml




Odoo - Sample 2 for three columns

B-BOX™ Blue Light LED Epi-illuminator

  • 470 nm long wavelength

  • Improved cloning efficiency 

  • Compact, light-weight, and portable (less than 1 kg)

  • Adjustable and removable filter plate allows for gel cutting, visualization, and documentation

Odoo - Sample 2 for three columns

GoPAGE™ Precast Gels

  • Enhances band sharpness

  • Numbered wells for easily loading samples

  • Two cassette sizes Compatible for most mini-gel tanks.

  • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference