[PM5100] ExcelBand™ 3-color Pre-Stained Protein Ladder, High Range (9-245 kDa), 250 μl x 2

Call For Price




 

Description 

The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. 


Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Three reference bands 75 kDa (red), 40 kDa (green), and 20 kDa (blue)


Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 


Quality Control 

Under suggested conditions, PM5100 ExcelBand™ 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 


Storage

4°C for 3 months
-20°C for long term storage 

 

Specification

Cat. No.

PM5100

Series Name

ExcelBand™

Product Size

250 μl x 2

MW Range

10 – 245 kDa

Band Number

14

Band Color

Red/Green/Blue

Markered Bands

75, 40, 20 kDa

 



Expression of Tumor Suppressor FHIT Is Regulated by the LINC00173-SNAIL Axis in Human Lung Adenocarcinoma

Takahito Suzuki, Satoshi Sakai, Kosuke Ota, Mika Yoshida, Chiharu Uchida, Hiroyuki Niida, Takafumi Suda, Masatoshi Kitagawa, Tatsuya Ohhata

Int J Mol Sci. 2023 Dec; 24(23): 17011. Published online 2023 Nov 30. doi: 10.3390/ijms242317011

PMCID: PMC10707390


Direct Detection of Pulmonary Fibrosis by Near-Infrared-Responsive Biomimetic Platelets

Guanlie Li, Haiqing He, Guodong Zheng, Wenjing Jiang, Shuwen Du, Hua Tao, Tao Xiao, Dazhi Zhou, Shangwei Ding, Xi-Yong Yu, Yu Zhang, Ao Shen

Int J Nanomedicine. 2022 Jan 10;17:151-162. doi: 10.2147/IJN.S334331. eCollection 2022.

PMCID: PMC8760469


Covalent conjugation of extracellular vesicles with peptides and nanobodies for targeted therapeutic delivery

Tin Chanh Pham, Migara Kavishka Jayasinghe, Thach Tuan Pham, Yuqi Yang, 2 Likun Wei, Waqas Muhammad Usman, Huan Chen, Marco Pirisinu, Jinhua Gong, Seongkyeol Kim, Boya Peng, Weixi Wang, Charlene Chan, Victor Ma, Nhung T.H. Nguyen, Dennis Kappei, Xuan‐Hung Nguyen, William C. Cho, Jiahai Shi,r and Minh T.N. Lecor 

J Extracell Vesicles. 2021 Feb; 10(4): e12057. Published online 2021 Feb 16. doi: 10.1002/jev2.12057

PMCID: PMC7886705


Efficient RNA drug delivery using red blood cell extracellular vesicles

Waqas Muhammad Usman, Tin Chanh Pham, Yuk Yan Kwok, Luyen Tien Vu, Victor Ma, Boya Peng, Yuen San Chan, Likun Wei, Siew Mei Chin, Ajijur Azad, Alex Bai-Liang He, Anskar Y. H. Leung, Mengsu Yang, Ng Shyh-Chang, William C. Cho, Jiahai Shi & Minh T. N. Le  

Nat Commun. 2018 Jun 15;9(1):2359. doi: 10.1038/s41467-018-04791-8.

PMCID: PMC6004015 


Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems