[PM5000] ExcelBand™ 3-color Pre-Stained Protein Ladder, Regular Range (9-180 kDa), 250 μl x 2

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Description 

The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.


Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)


Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 


Quality Control 

Under suggested conditions, PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range resolves 13 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 


Storage

4°C for 3 months 
-20°C for long term storage

 

Specification

Cat. No.

PM5000

Series Name

ExcelBand™

Product Size

250 μl x 2

MW Range

10 – 180 kDa

Band Number

13

Band Color

Red/Green/Blue

Markered Bands

75, 40, 20 kDa

 



Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability

Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011

PMCID: PMC4709241

Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems