[PM5000] ExcelBand™ 3-color Pre-stained Protein Ladder, Regular Range (9-180 kDa), 250 μl x 2

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Description 

The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.


Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)


Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 


Quality Control 

Under suggested conditions, PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range resolves 13 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. 


Storage

4°C for 3 months 
-20°C for 24 months

 

Specification

Cat. No.

PM5000

Series Name

ExcelBand™

Product Size

2 x 250 μl

MW Range

10 – 180 kDa

Band Number

13

Band Color

Red/Green/Blue

Markered Bands

75, 40, 20 kDa

 


 

Unraveling the novel effects of aroma from small molecules in preventing hen egg white lysozyme amyloid fibril formation

Zahra Seraj, Arefeh Seyedarabi, Ali Akbar Saboury, Mehran Habibi-Rezaei, Shahin Ahmadian, Atiyeh Ghasemi PLoS One. 2018; 13(1): e0189754. Published online 2018 Jan 22. doi: 10.1371/journal.pone.0189754

PMCID: PMC5777642


The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971

PMCID: PMC5790263


Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability

Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011

PMCID: PMC4709241

Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

GoPAGE™ Precast Gels

User-friendly gel cassette:

  • Easy to use— No comb or tape to remove. 

  • Easy to load samples— Numbered wells; extended and fixed well separator to prevent sample carryover.

  • Easy to monitor— Transparent reference lines on the gel cassette help to monitor electrophoresis.

Unique gel formula: 

  • Sharpness— Enhances band sharpness

  • Long shelf life— Up to 12 months when stored at 4°C

Broad compatibility: 

  • Wide separation range— Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

  • Compatibility— Two cassette sizes suitable for most mini-gel tanks.