[PM2510] ExcelBand™ Enhanced 3-color Regular Range Protein Marker (9-180 kDa), 250 μl x 2

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Description 

The PM2510 ExcelBand™ Enhanced 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). PM2510 ExcelBand™ Enhanced 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.


Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Two reference bands — 75 kDa (red) and 25 kDa (green)


Contents 

Approximately 0.2~0.6 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 3.6 M urea, and 15% (v/v) glycerol).


Quality Control

Under suggested conditions, PM2510 ExcelBand™ Enhanced 3-color Regular Range Protein Marker resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 


Storage 

4°C for 3 months
-20°C for long term storage

 

Specification

Cat. No.

PM2510 / PM2511

Series Name

ExcelBand™

Product Size

250 μl x 2 / 250 μl x 10

MW Range

10 – 180 kDa

Band Number

10

Band Color

Red/Green/Blue

Markered Bands

25, 75 kDa

 


 

A jacalin-related lectin domain-containing lipase from chestnut (Castanea crenata): Purification, characterization, and protein identification 

Jun Heo, Chang Woo Kwon, Juno Lee, Haena Park, Hyunjong Yu, and Pahn-Shick Chang

Curr Res Food Sci. 2022; 5: 2081–2093. Published online 2022 Nov 2. doi: 10.1016/j.crfs.2022.10.033

PMCID: PMC9641177


Purification and Characterization of a Dark Red Skin Related Dimeric Polyphenol Oxidase from Huaniu Apples

Bin Liu, Xianfang Zhou, Haiyan Guan, Xuequn Pang and Zhaoqi Zhang,  Foods. 2022 Jun 17;11(12):1790. doi: 10.3390/foods11121790.

PMCID: PMC9223062


Butein Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers

Shuangying Chao, Yuhang Liu, Ning Ding, Yue Lin, Qian Wang, Junwen Tan, Wei Li, Yang Zheng, Xuejun Hu, Junming Li,  Front Mol Biosci

. 2022 Mar 10;9:848829. doi: 10.3389/fmolb.2022.848829. eCollection 2022.

PMCID: PMC8960375


Novel Butein Derivatives Repress DDX3 Expression by Inhibiting PI3K/AKT Signaling Pathway in MCF-7 and MDA-MB-231 Cell Lines

Shailima Rampogu, Seong Min Kim, Baji Shaik, Gihwan Lee, Ju Hyun Kim, Gon Sup Kim, Keun Woo Lee, Myeong Ok Kim, Front Oncol. 2021 Aug 18;11:712824. doi: 10.3389/fonc.2021.712824. eCollection 2021.

PMCID: PMC8416463


Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems