[PM1500] ExcelBand™ All Blue Regular Range Protein Marker (9-180 kDa), 250 μl x 2

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Description 

The PM1500 ExcelBand™ All Blue Regular Range Protein Marker is a blue protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).

The PM1500 ExcelBand™ All Blue Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.


Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Two enhanced bands — 72 kDa and 25 kDa


Contents

Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 


Quality Control

Under suggested conditions, PM1500 ExcelBand™ All Blue Regular Range Protein Marker resolves 10 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 


Storage

4°C for 3 months
-20°C for long term storage 

 

Specification

Cat. No.

PM1500

Series Name

ExcelBand™

Product Size

250 μl x 2

MW Range

10 – 180 kDa

Band Number

10

Band Color

All blue

Enhanced Bands

25, 72 kDa



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Ulpiyana A, Frima FK, Annisa DS, Tan JC, Puspasari F, Aditama R, Ihsanawati, Natalia D.

Heliyon. 2024 Jul 5;10(14):e33667. doi:10.1016/j.heliyon.2024.e33667. eCollection 2024 Jul 30.

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J Am Chem Soc. 2024 Feb 14; 146(6): 4087–4097. Published online 2024 Jan 31. doi: 10.1021/jacs.3c12668

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α-1,3-Glucanase from the gram-negative bacterium Flavobacterium sp. EK-14 hydrolyzes fungal cell wall α-1,3-glucan

Masaki Takahashi, Shigekazu Yano, Yui Horaguchi, Yuitsu Otsuka, Wasana Suyotha, Koki Makabe, Hiroyuki Konno, Susumu Kokeguchi

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PMCID: PMC10696023


Unraveling the novel effects of aroma from small molecules in preventing hen egg white lysozyme amyloid fibril formation

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The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

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Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011

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Comparative biochemical analysis of full-length and truncated BaqA alpha-amylases from Bacillus aquimaris MKSC 6.2.

Ulpiyana A, Frima FK, Annisa DS, Tan JC, Puspasari F, Aditama R, Ihsanawati, Natalia D.

Heliyon. 2024 Jul 5;10(14):e33667. doi:10.1016/j.heliyon.2024.e33667. eCollection 2024 Jul 30.


Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA–Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element

Erika Komiya, Shouhei Takamatsu, Daimei Miura, Kaori Tsukakoshi, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro, Ryutaro Asano

J Am Chem Soc. 2024 Feb 14; 146(6): 4087–4097. Published online 2024 Jan 31. doi: 10.1021/jacs.3c12668

PMCID: PMC10870700


α-1,3-Glucanase from the gram-negative bacterium Flavobacterium sp. EK-14 hydrolyzes fungal cell wall α-1,3-glucan

Masaki Takahashi, Shigekazu Yano, Yui Horaguchi, Yuitsu Otsuka, Wasana Suyotha, Koki Makabe, Hiroyuki Konno, Susumu Kokeguchi

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PMCID: PMC5777642


The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971

PMCID: PMC5790263


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Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011

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Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems