【IT1100】EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP), 50 RXN

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Description

The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.


Features

  • High yield

  • Versatile- suitable for short and long transcripts

  • NTP premixed- Minimal pipetting and setup time

  • Compatible with CleanCap® Reagent AG

  • Lithium chloride included for RNA purification


Application

  • Generation of RNA from T7 promoter-driven DNA sequences

  • Suitable for subsequent cap-0 and cap-1 modification


Storage

-20°C for 12 months


Sample 1 for three columns

 Time course of RNA synthesis from 3 DNA templates

The 0.2k, 2k and 8k RNA transcripts were generated using the SMOBIO EzRNA™T7 High Yield RNA Synthesis Kit, the reaction containing 1 ug DNA template was incubated at 37°C for indicated reaction time.

Sample 2 for three columns

Dose effect of DNA template on RNA yield 

The 0.2k and 2k RNA transcripts were generated using the SMOBIO EzRNA™T7 High Yield RNA Synthesis Kit , the reaction containing indicated amounts of DNA template was incubated at 37°C for 2 hours.

Sample 3 for three columns

Yield of RNA synthesized by EzRNA™ T7 High Yeield RNA Synthesis Kits    

Contents

Components

Volume

T7 RNA Polymerase Mix

100 μl

10X T7 Reaction Buffer

100 μl

NTP (Ψ) Premix (25 mM each)

400 μl

Control DNA (0.5 μg/μl)

10 μl

Lithium Chloride (7.5M)

ml

Nuclease-Free Water

ml


Storage

-20°C for 12 months

1. The Control DNA is approximately 2,110 base pairs in length and is provided as linearized DNA, which can be used directly in the IVT reaction without any additional                        processing. The RNA produced in the IVT reaction will typically have the same length as the DNA template, around 2,110 nucleotides.

2. The concentration of the Control DNA is 0.5 μg/μL. For a standard IVT reaction, we recommend using 1 μg of DNA (approximately 2 μL). However, if you need to use the                Control DNA more frequently, you can reduce the amount used per reaction (e.g., 0.5 μg) to extend the number of uses, while still maintaining experimental reliability.


  1. Before setting up the reactions, please gently mix the components with a pipette and centrifuge briefly to collect at the bottom of the tube.

  2. Please prepare the reaction at room temperature: When preparing the reaction mixture, except for the T7 RNA Polymerase Mix, which should be temporarily stored on ice, other components should be kept at room temperature, and the reaction should be prepared at room temperature.

  3. Please include each reaction component following this sequence: Water → Reaction Buffer → NTP Premix → DNA template → T7 RNA Polymerase. Ensure that the template and enzyme are added last.

  4. Make sure reactions are thoroughly mixed prior to subject to 37℃.

  5. To avoid volatilization of the reaction solution due to prolonged transcription, we recommend incubating the IVT reactions in a PCR machine or in a dry air incubator.

Odoo - Sample 1 for three columns

[RC1000] EzRNA™ RNA Capping System, 50 RXN 

Application
  • Generation of 5’Cap-0 (m7Gppp) and Cap-1 (m7GpppNm-) RNA by enzymatic reaction

  • mRNA synthesis for in vitro translation

  • Gene expression studies

  • mRNA vaccine development and therapeutics

Odoo - Sample 1 for three columns

[IT1000] EzRNA™T7 High Yield RNA Synthesis Kit, 50 RXN

Application

  • Generation of RNA from T7 promoter-driven DNA sequences

  • Suitable for subsequent cap-0 and cap-1 modification

Odoo - Sample 1 for three columns

[IT1200] EzRNA™ T7 High Yield RNA Synthesis Kit (me1Ψ-UTP), 50 RXN

Application

  • Generation of RNA from T7 promoter-driven DNA sequences

  • Suitable for subsequent cap-0 and cap-1 modification


 

Odoo - Sample 1 for three columns

[RI1000] RNAok™ RNase Inhibitor

Application

  • RT-PCR

  • cDNA Synthesis 

  • in vitro transcription

 

Odoo - Sample 3 for three columns

FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng (DNA) or or 1 ng (total RNA)

  • A safe alternative to EtBr

  • Suitable for blue or UV light