[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl

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Description

The FluoroStain™ DNA Fluorescent Staining Dye is designed to be a safer replacement for conventional Ethidium bromide (EtBr) which poses a significant health and safety hazard for its users. The FluoroStain™ DNA Fluorescent Staining Dye offers at least 10 times sensitivity in DNA detection levels, and is capable of detecting double stranded DNA (dsDNA) fragments up to 0.04 ng in electrophoresis analysis. The FluoroStain™ DNA Fluorescent Staining Dye shows a high specificity to the dsDNA, with negligible background signal, making the destaining process entirely optional. FluoroStain™ DNA Fluorescent Staining Dye is compatible with both the conventional ultra violet gel-illuminating systems as well as the less harmful long wave length blue light illumination systems. The emission when bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Features:

  • Excellent for post staining

  • Sensitivity: 0.04 ng DNA

  • A safer alternative to EtBr

  • Compatibility: suitable to blue or UV light

  • Increased cloning efficiency (blue light)

 

Storage

Protected from light
4°C for 12 months
-20°C for 24 months

Odoo - Sample 1 for three columns

Sensitivity of FluoroStain™

The FluoroStain™ DNA Fluorescent Staining Dye (DS1000) shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. 

Odoo - Sample 2 for three columns

Excitation and emission spectrum of FluoroStain™ 

FluoroStain™ DNA Fluorescent Staining Dye (DS1000) emission as bound to dsDNA is 522 nm while its excitation peaks are at 270, 370 and 497 nm. 

Odoo - Sample 3 for three columns

Non-mutagenicity of FluoroStain™

FluoroStain™ DNA Fluorescent Staining Dye (DS1000) is proofed for their safety (non-mutagenicity) using Ames test. However, it must be noted that since solvent may penetrate the skin, it is recommended that users wear gloves when using the fluorescent dyes.

 Contents

Component

Volume

Cat. No.

FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X)

500 μl

DS1000

FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X)

5 x 500 μl

DS1001

 

Storage

Protected from light
4°C for 12 months
-20°C for 24 months

To avoid the solid precipitation of FluoroStain™ DNA Fluorescent Staining Dye (DS1000) during -20℃ (4℃) storage, we recommend opening the storage tubes only when the DS1000 solution is complete melted.

If solid precipitation of DS1000 was observed, warm the tube at 37℃ for 10 min and then vortex to dissolve the solid particles. If the solid precipitation of DS1000 was still unable to be completely dissolved, spin down the precipitate and use only supernatant to dilute in TAE/TBE/TE buffer. Few solid precipitation of DS1000 will not cause the decline of sensitivity.


 

Before opening

Warm the vial to an ambient temperature until the solution is thawed thoroughly.

Vortex and spin down the content of the vial to ensure the solution is homogeneous.

Working Reagent Preparation

1:10,000 dilution in TE, TAE or TBE buffer

Odoo - Sample 1 for three columns

Staining after electrophoresis (post-staining)

This protocol is highly recommended.

1. Performing agarose gel electrophoresis following your standard protocol.

 2.Dilute FluoroStain™ DNA Fluorescent Staining Dye reagent 10,000 folds in a TE, TAE, or TBE buffer.

For example: 10 μl in 100 ml TAE buffer 

Use a plastic container. Glass containers are not recommended, as they absorb fluorescent dye in staining solution. 

Protect the staining container from light by covering it with aluminium foil, or place it in the dark. 

The staining solution can be stored for up to one week or more.

3. Immerse the gel in a staining solution (1X) and incubate at room temperature for 10 - 30 minutes. (avoid light).

Staining time varies with the thickness of the gel and percentage of agarose. If needed, agitate the gel gently at room temperature to shorten staining time. 

De-staining is not required. 

4. Visualize or photograph the gel with UV or blue-light illumination (blue-light is recommended).

Clean the surface of the illuminator before and after each use with deionized water. Accumulation of fluorescent dyes on the surface will create a high fluorescent background.

Video cameras and CCD cameras have a different spectral response compared to the black-and-white print film and therefore may not exhibit the same sensitivity.

Odoo - Sample 3 for three columns

B-BOX™ Blue Light LED Epi-illuminator

  • 470 nm long wavelength

  • Improved cloning efficiency 

  • Compact, light-weight, and portable (less than 1 kg)

  • Adjustable and removable filter plate allows for gel cutting, visualization, and documentation

Odoo - Sample 1 for three columns

ExcelBand™ DNA Ladder series

  • Sharp bands

  • Quick reference enhanced bands 

  • Ready-to-use— premixed with loading dye for direct loading

  • Stable— room temperature storage over 6 months

Odoo - Sample 3 for three columns

FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng (DNA) or or 1 ng (total RNA)

  • A safe alternative to EtBr

  • Suitable for blue or UV light