[DM3160] FluoroBand™ 1 KB (0.25-10 kb) Fluorescent DNA Ladder, 500 μl
The DM3160 FluoroBand™ 1KB (0.25-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA ladder DM3160 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
250 ~ 10,000 bp
50 µg/ 500 µl
Recommended loading volume
5 µl/ well
Protected from light
Room temperature for 6 months
4°C for 12 months
-20°C for 24 months
250 – 10000 bp
1000 and 3000 bp
Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?
Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.
Can I combine non-fluorescent markers and fluorescent loading dye (ex. DL5000) to replace FluoroBand™ fluorescent DNA ladders?
SMOBIO’s fluorescent DNA ladders is better in intensity and accuracy than a fluorescent marker produced by mixing non-fluorescent one with reagents containing fluorescent DNA dyes such as DL5000. In another aspect, DL5000 is designed for quick screening, not intended for preparation of DNA ladders which needs careful calibration. Therefore we suggest using our fluorescent DNA ladders directly.
Will FluoroBand™ fluorescent DNA ladders gradually lose fluorescent intensity?
The fluorescent signals of a fluorescent DNA ladder might be reduced if frequently exposed to light for a long term. Therefore, we suggest keeping fluorescent DNA ladders from exposure to light.
Can fluorescent DNA markers be visualized when illuminated with UV light?
Yes, it is possible to view the fluorescent signals under blue light and UV light.
FluoroDye™ DNA Fluorescent Loading Dye
Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Convenience - monitor the electrophoresis in real-time
FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
B-BOX™ Blue Light LED Epi-illuminator
470 nm long wavelength
Improved cloning efficiency
Compact, light-weight, and portable (less than 1 kg)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation