[CD7000] SMOChem™ dUTP Solution - Sodium Salt (100 mM)

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Description

Ultrapure dUTP (2´-Deoxyuridine, 5´-Triphosphate) supplied as sodium salt in purified water (pH 8.5).  dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity. 


Features

  • Ideal for PCR amplification and cDNA synthesis

  • Nuclease and ribonuclease free


Applications

  • DNA amplification

  • Avoid carryover contamination between PCRs to eliminate a source of false positives.


Storage

-20°C for 24 months

Odoo - Sample 1 for three columns

Ultrapure 

Greater than 99% purity confirmed by HPLC

Odoo - Sample 3 for three columns

Highly Stable

The purity of SMOBIO’s dNTP remained greater than 99% after one week storage at 25°C.

Specification

Product Name

dATP Solution - Sodium Salt

dTTP Solution - Sodium Salt

dCTP Solution - Sodium Salt

dGTP Solution - Sodium Salt

dUTP Solution - Sodium Salt

Cat. No.

CD3000

CD4000

CD5000

CD6000

CD7000

Nomenclature

2’-Deoxyadenosine

5’-triphosphate

2’-Deoxythymidine

5’-triphosphate

2’-Deoxycytidine

5’-triphosphate

2’-Deoxyguanosine

5’-triphosphate

2’-Deoxyuridine

5’-triphosphate

Formula (Anion)

C10H13N5O12P3

C10H14N2O14P3

C9H13N3O13P3

C10H13N5O13P3

C9H12N2O14P3

Storage

at -20 °C

at -20 °C

at -20 °C

at -20 °C

at -20 °C

Stability

24 months

24 months

24 months

24 months

24 months

Appearance

Colorless

Colorless

Colorless

Colorless

Colorless

Concentration

100-110mM

100-110mM

100-110mM

100-110mM

100-110mM

Purity (HPLC)

 99%

 99%

 99%

 99%

 99%

A250/A260

0.78±0.02

0.64±0.02

0.82±0.02

1.15±0.03

0.74±0.03

A280/A260

0.15±0.01

0.74±0.02

0.97±0.02

0.67±0.02

0.38±0.02

A290/A260

N/A

0.24±0.02

0.30±0.02

0.28±0.02

N/A

pH (4°C)

8.5±0.1

8.5±0.1

8.5±0.1

8.5±0.1

8.5±0.1

Cation (Mg2+)

 5 mM

 5 mM

 5 mM

 5 mM

 5 mM

Phosphate (PO43-)

 2 mM

 2 mM

 2 mM

 2 mM

 2 mM

Contamination with human DNA (18S rRNA) or bacterial DNA (16S rRNA gene)

Negative

Negative

Negative

Negative

Negative

Proteases, DNases, RNases, or Nicking Activity

Negative

Negative

Negative

Negative

Negative


 

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (20 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 20 μl dUTP to 50 μl water, PCR grade).


  

Product Description

Size

Cat. No.

dATP Solution - Sodium Salt (100 mM) 

25 ml

CD3000

100 ml

CD3001

dTTP Solution - Sodium Salt (100 mM) 

25 ml

CD4000

100 ml

CD4001

dCTP Solution - Sodium Salt (100 mM) 

25 ml

CD5000

100 ml

CD5001

dGTP Solution - Sodium Salt (100 mM) 

25 ml

CD6000

100 ml

CD6001

dUTP Solution - Sodium Salt (100 mM) 

25 ml

CD7000

100 ml

CD7001

Deoxynucleotide (dNTP) Mix, 10 mM each (40 mM total) 

200 µl

CD1010

200 µl x 5

CD1011

Deoxynucleotide (dNTP) Mix, 25 mM each (100 mM total)  

500 µl

CD1020

500 µl x 6

CD1021