Description
Ultrapure dATP supplied as sodium salt in purified water (pH 8.5).
Features
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Applications
PCR
A-Tailing with Taq DNA Polymerase
Storage
-20°C for 36 months
Ultrapure
Greater than 99% purity confirmed by HPLC.
Highly Stable
The purity of SMOBIO’s dNTP remained greater than 99% after one week storage at 25°C.
Functionality
SMOBIO’s dNTP are optimal performance for general PCR (A), long range PCR (B), RT-PCR (C), and real-time PCR (D).
Specification
Product Name | dATP Solution - Sodium Salt | dTTP Solution - Sodium Salt | dCTP Solution - Sodium Salt | dGTP Solution - Sodium Salt | dUTP Solution - Sodium Salt |
Cat. No. | CD3000 | CD4000 | CD5000 | CD6000 | CD7000 |
Nomenclature | 2’-Deoxyadenosine 5’-triphosphate | 2’-Deoxythymidine 5’-triphosphate | 2’-Deoxycytidine 5’-triphosphate | 2’-Deoxyguanosine 5’-triphosphate | 2’-Deoxyuridine 5’-triphosphate |
Formula (Anion) | C10H13N5O12P3 | C10H14N2O14P3 | C9H13N3O13P3 | C10H13N5O13P3 | C9H12N2O14P3 |
Storage | at -20 °C | at -20 °C | at -20 °C | at -20 °C | at -20 °C |
Stability | 24 months | 24 months | 24 months | 24 months | 24 months |
Appearance | Colorless | Colorless | Colorless | Colorless | Colorless |
Concentration | 100-110mM | 100-110mM | 100-110mM | 100-110mM | 100-110mM |
Purity (HPLC) | ≧ 99% | ≧ 99% | ≧ 99% | ≧ 99% | ≧ 99% |
A250/A260 | 0.78±0.02 | 0.64±0.02 | 0.82±0.02 | 1.15±0.03 | 0.74±0.03 |
A280/A260 | 0.15±0.01 | 0.74±0.02 | 0.97±0.02 | 0.67±0.02 | 0.38±0.02 |
A290/A260 | N/A | 0.24±0.02 | 0.30±0.02 | 0.28±0.02 | N/A |
pH (4°C) | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 |
Cation (Mg2+) | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM |
Phosphate (PO43-) | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM |
Contamination with human DNA (18S rRNA) or bacterial DNA (23S rRNA) | Negative | Negative | Negative | Negative | Negative |
Proteases, DNases, RNases, or Nicking Activity | Negative | Negative | Negative | Negative | Negative |