1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Tris-Glycine Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.
Reliable: Rigorous quality control for reproducible separation of protein electrophoresis.
Convenient: Premeasured pouches make 1 liter of 1X buffer solution; No pH adjustment is necessary.
Fast: Dissolving in minutes and then ready to use.
Stable: Powder packaging suitable for long-term storage.
0.025 M Tris, 0.192 M glycine, 0.10% SDS
Running buffer for Laemmli Tris-HCl gel electrophoresis
What is the intended use of Tris-Glycine-SDS electrophoresis buffer?
Tris-Glycine-SDS Running Buffer is formulated for separating denatured proteins on Laemmli Tris-HCl gels in Tris-Glycine buffer system. Laemmli Tris-HCl gels provide reproducible separation of a wide range of proteins into well-resolved bands.
What is the function of SDS in the Tris-Glycine-SDS buffer?
SDS (sodium dodecyl sulfate) is an anionic detergent that denatures proteins and coats proteins with negative charge. The net negative charge it imparts when it binds to protein causes repulsion between amino acids. It is added in excess to the proteins, so that a similar charge-to-mass ratio is obtained for all proteins. Therefore, the migration rate of proteins will be only dependent on their size, but not their intrinsic charge.
Can I reuse Tris-Glycine-SDS running buffer?
Always use fresh buffer to make the gel running properly. Reuse Tris-Glycine-SDS running buffer may change pH value, increase ion concentration, and contaminate protein analytes by residual proteins, which may interfere with the electrophoresis effect.
Do I have to use deionized water to prepare the 1X Tris-Glycine-SDS running buffer?
Yes, please use deionized water and make sure the powder is completely dissolved before use. Using water with ions, such as mineral water, may cause turbidity in running buffer, which may affect electrophoresis results.
Can I use only half of a pack to make a 500 ml of 1X Tris-Glycine-SDS running buffer?
No, please dissolve one pack of 1X Tris-Glycine-SDS Running Buffer Powder in 1L deionized water to make 1L 1X Tris-Glycine-SDS running buffer at a time. Split use of a single pack may cause changes in buffer composition or pH value due to uneven powder distribution, which may affect electrophoresis results.
The appearance of SMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder looks like fragmented noodles, is it normal?
Yes, it is normal. The appearance and traits of SDS included in the SMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder is white fragmented noodles rather than fine powder.