[BF4000] SMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder, 5 packs (For 5000 ml)

Tris-Glycine-SDS Running Buffer is formulated for separating denatured proteins on Laemmli Tris-HCl gels in Tris-Glycine buffer system. Laemmli Tris-HCl gels provide reproducible separation of a wide range of proteins into well-resolved bands.

SDS (sodium dodecyl sulfate) is an anionic detergent that denatures proteins and coats proteins with negative charge. The net negative charge it imparts when it binds to protein causes repulsion between amino acids. It is added in excess to the proteins, so that a similar charge-to-mass ratio is obtained for all proteins. Therefore, the migration rate of proteins will be only dependent on their size, but not their intrinsic charge.

Always use fresh buffer to make the gel running properly. Reuse Tris-Glycine-SDS running buffer may change pH value, increase ion concentration, and contaminate protein analytes by residual proteins, which may interfere with the electrophoresis effect.

Yes, please use deionized water and make sure the powder is completely dissolved before use. Using water with ions, such as mineral water, may cause turbidity in running buffer, which may affect electrophoresis results.

No, please dissolve one pack of 1X Tris-Glycine-SDS Running Buffer Powder in 1L deionized water to make 1L 1X Tris-Glycine-SDS running buffer at a time. Split use of a single pack may cause changes in buffer composition or pH value due to uneven powder distribution, which may affect electrophoresis results.