1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system.
Reliable: Rigorous quality control for reproducible separation of protein electrophoresis.
Convenient: Premeasured pouches make 1 liter of 1X buffer solution; No pH adjustment is necessary.
Fast: Dissolving in minutes and then ready to use.
Stable: Powder packaging suitable for long-term storage.
50mM Tris Base, 50mM MOPS, 0.1% SDS, 1mM EDTA
Running buffer for sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) in Bis-Tris buffer system
What is the intended use of MOPS-SDS electrophoresis buffer?
MOPS-SDS Running Buffer has been used as a running buffer for sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) in Bis-Tris buffer system. As compared with MES-SDS running buffer, this buffer is suitable for separating medium-to-large- sized proteins.
Can I reuse MOPS-SDS running buffer?
Always use fresh buffer to make the gel running properly. Reuse MOPS-SDS running buffer may change pH value, increase ion concentration, and contaminate protein analytes by residual proteins, which may interfere with the electrophoresis effect.
Do I have to use deionized water to prepare the 1X MOPS-SDS running buffer?
Yes, please use deionized water and make sure the powder is completely dissolved before use. Using water with ions, such as mineral water, may cause turbidity in running buffer, which may affect electrophoresis results.
Can I use only half of a pack to make a 500 ml of 1X MOPS-SDS running buffer?
No, please dissolve one pack of 1X MOPS-SDS Running Buffer Powder in 1L deionized water to make 1L 1X MOPS-SDS running buffer at a time. Split use of a single pack may cause changes in buffer composition or pH value due to uneven powder distribution, which may affect electrophoresis results.