[TP2160] ExcelTaq™ 5X Blood Direct Fluoro PCR Kit, 200 Rxn

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Description

The ExcelTaq™ 5X Blood Direct Fluoro PCR Kit is designed for amplifying target DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ 5X Blood Direct Fluoro PCR Kit contains 5X concentrated mixture of all the essential components of a PCR reaction. This master mix also contains a PCR-friendly loading and tracking dye (Orange G), as well as a safer fluorescent DNA staining dye. The resultant PCR reaction mixture can be loaded directly into gels for electrophoresis. The included fluorescent DNA staining dye allows users to track electrophoresis process in real time and helps to eliminate the need for staining process. ExcelTaq™ 5X Blood Direct Fluoro PCR Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. Therefore, the ExcelTaq™ 5X Blood Direct Fluoro PCR Kit is ideal with high throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct Fluoro PCR Kit includes a pairs of Positive Control Primers (CCR5) that are compatible with primate blood samples. 


Features

  • 5'→3' DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs 

  • High throughput PCR

  • High yield PCR 

  • High reproducibility, with reduced  pipetting errors

  • Compatible with most anticoagulants

  • DNA bands can be visualized directly under UV or 470 nm blue light illuminations.


Applications 

  • Direct amplification of DNA from blood samples

  • High throughput screening without DNA purification

  • Suitable for multiplex PCR


Storage

Protected from light and avoid multiple freeze/thaw cycles

4°C for 6 months
-20°C for 24 months


Odoo - Sample 1 for three columns

Compatible with most anticoagulants 

ExcelTaq™ 5X Blood Direct Fluoro PCR Mix amplified 200 bp from differently treated blood using CCR5 specific primers. (M: DM2160)

Contents

Component

Volume

5X Blood Direct Fluoro PCR Mix

2 x 1 ml

Positive Control Primers (10 μM, each)

25 μl


CCR5 control primer sequence

CCR5-F: 5'-CTCCCAGGAATCATCTTTACC-3'

CCR5-R: 5'-TCATTTCGACACCGAAGCAG-3'


Storage

Protected from light and avoid multiple freeze/thaw cycles

4°C for 6 months
-20°C for 24 months

 

Recommended PCR Condition

Blood

3 μl

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

5X Blood Direct Fluoro PCR Mix

10 μl

H2O

to 50 μl

Total volume

50 µl

Note: Mix well before PCR Reaction

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

3 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C**

30 sec

Extension

72°C

60 sec/kb

Final extension

72°C

3 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.

 

Post amplification analysis

Centrifuge PCR reaction mixture at 1000 × g for 1 ~ 3 minutes. Load only the clear supernatant of the PCR products for gel electrophoresis analysis.

 

Odoo - Sample 3 for three columns

B-BOX™ Blue Light LED Epi-illuminator

  • 470 nm long wavelength

  • Improved cloning efficiency 

  • Compact, light-weight, and portable (less than 1 kg)

  • Adjustable and removable filter plate allows for gel cutting, visualization, and documentation

Odoo - Sample 1 for three columns

FluoroBand™ DNA Ladder series

  • Sharp bands

  • Quick reference enhanced bands 

  • Ready-to-use— premixed with loading dye for direct loading

  • Stable— room temperature storage over 6 months

  • Directly observed by UV or blue light— premixed with high sensitive fluorescent dye

Odoo - Sample 3 for three columns

FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng (DNA) or or 1 ng (total RNA)

  • A safe alternative to EtBr

  • Suitable for blue or UV light