[TP1200/TP1210/TP1220] ExcelTaq™ PCR Master Dye Mix

Call For Price




  

Description

The ExcelTaq™ 5X/2X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X/2X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl(TP1220 contains MgSO4), dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.

 

Features

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR


Applications 

  • Routine PCR

  • Colony PCR

  • High throughput PCR

  • Amplification of DNA fragments up to 8 kb

  • Generation of PCR products for TA cloning

  • DNA labeling


Storage

4°C for 6 months
-20°C for 24 months

Odoo - Sample 1 for three columns

Elongation capability

ExcelTaq™ PCR Master Mix can reliably amplify λDNA up to 8 kb in length. (M: DM3100)

 

Contents

Component

Volume

Cat. No

ExcelTaq™ 5X PCR Master Dye Mix

2 x 1 ml

TP1200 (200 Rxn)

ExcelTaq™ 2X PCR Master Dye Mix

2 x 1.25 ml

TP1210 (100 Rxn)

ExcelTaq™ 2X PCR Master Dye Mix (MgSO4)

2 x 1.25 ml

TP1220 (100 Rxn)


Storage

4°C for 6 months
-20°C for 24 months

  

Recommended PCR Condition

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

5X (or 2X) PCR Master Dye Mix

10 µl (or 25 µl)

H2O

to 50 µl

Total volume

50 µl



Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C*

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.

Odoo - Sample 1 for three columns

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

Odoo - Sample 3 for three columns

Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.