[TP1100/TP1110/TP1120] ExcelTaq™ PCR Master Mix

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Description 

The ExcelTaq™ 5X/2X PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of templates and primers. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5X/2X concentrated ready-to-use mixture of recombinant Taq DNA Polymerase, reaction buffer, MgCl2 (TP1120 contains MgSO4), dNTP, and enzyme stabilizer enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent conveniently. This product is supplied with 6X DNA Loading Dye (Blue) containing two tracking dyes (Xylene cyanol FF and Bromophenol blue) for post PCR analysis through the use of agarose gel electrophoresis.


Features

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced pipetting errors


Applications 

  • Routine PCR

  • Colony PCR

  • High throughput PCR

  • Amplification of DNA fragments up to 8 kb

  • Generation of PCR products for TA cloning

  • DNA labeling


Storage

4°C for 6 months
-20°C for 24 months

Odoo - Sample 1 for three columns

Elongation capability

ExcelTaq™ PCR Master Mix can reliably amplify λDNA up to 8 kb in length. (M: DM3100)

 

Contents

Component

Volume

Cat. No

ExcelTaq™ 5X PCR Master Mix

2 x 1 ml

TP1100 (200 Rxn)

6X DNA Loading Dye (Blue)

2 x 1 ml

ExcelTaq™ 2X PCR Master Mix

2 x 1.25 ml

TP1110 (100 Rxn)

6X DNA Loading Dye (Blue)

1 ml

ExcelTaq™ 2X PCR Master Mix (MgSO4)

2 x 1.25 ml

TP1120 (100 Rxn)

6X DNA Loading Dye (Blue)

1 ml


Storage

4°C for 6 months
-20°C for 24 months

 

Recommended PCR Condition

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

5X (or 2X) PCR Master Mix

10 µl (or 25 µl)

H2O

to 50 µl

Total volume

50 µl


Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C*

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.

 

Odoo - Sample 1 for three columns

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

Odoo - Sample 2 for three columns

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   

Safe fluorescent dyes

Blue-light illuminator

Odoo - Sample 3 for three columns

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.  

Odoo - Sample 3 for three columns

Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.