[RP1100] ExcelRT™ 2X One-Step RT-PCR Kit, 50 RXN

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Description 

The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis 


Features

  • Generates dsDNA directly from RNA samples 

  • High sensitivity and yields 

  • High reproducibility, reduced pipetting errors

  • High throughput 

  • Reduced RNase H ribonuclease activity


Storage

-20°C for 24 months 

 

Contents

Component

Volume

Taq /RT Enzyme Mix

50 μl

2X One-Step Buffer

2 x 750 μl


Storage

-20°C for 24 months 

Odoo - Sample 1 for three columns

High sensitivity and yields 

 

Odoo - Sample 1 for three columns

[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

Odoo - Sample 3 for three columns

[RI1000] RNAok™ RNase Inhibitor

Application

  • RT-PCR

  • cDNA Synthesis 

  • in vitro transcription

Odoo - Sample 2 for three columns

[NS1000] FluoroVue™ Nucleic Acid Gel Stain

  • Excellent for in-gel staining

  • Sensitivity up to 0.14 ng DNA or 1 ng total RNA

  • A safer alternative to EtBr 

  • Suitable to blue or UV light 

Odoo - Sample 3 for three columns

[RQ2110] ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)

  • High yield

  • Reverse transcription at wide temperature range (42-60°C)

  • High specificity 

  • Suitable for fast cycle program

  • With ROX reference dye